Genome binding/occupancy profiling by high throughput sequencing Expression profiling by high throughput sequencing Other
Summary
We found that the CTCF binding site (named as EACBE) at the enhancer Eα of Tcra is essential for normal Tcra rearrangement.The EACBE deletion impaired the TAD structure of the 3’ region of the Tcra/d locus and the interactions between the proximal Vα genes and the Jα genes without changing the accessibility of the Jα array.
Overall design
Histone modification, repertoire and chromatin conformation profiles of 4-8 weeks old wild type (WT) and EACBE-/- mice were generated by deep sequencing, in duplicate, using Illumina Hiseq.