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Series GSE14517 Query DataSets for GSE14517
Status Public on Mar 30, 2009
Title Buffering of segmental and chromosomal aneuploidies in Drosophila melanogaster
Organism Drosophila melanogaster
Experiment type Expression profiling by array
Summary Chromosomal instability which involves deletion and duplication of chromosomes or chromosome parts is a common feature of cancers, and deficiency screens are commonly used as a method to find genes involved in different biological pathways. Still, how gene expression from whole chromosomes or large chromosomal domains is affected by deficiencies, duplications or chromosome loss is largely unknown. Using expression microarrays of deficiency hemizygotes and a duplication hemizygote we show that expressed genes are significantly buffered when present in a deficiency hemizygote and that the buffering effect is general and not mainly caused by feedback regulation of individual genes. Differentially expressed genes are in general better buffered than ubiquitously expressed genes when present in one copy. When present in three copies, differentially expressed genes are in general less buffered than ubiquitously expressed genes. Furthermore, we show that the 4th chromosome is compensated in response to dose differences. Our results suggest that general mechanisms exist to stimulate and to repress gene expression of aneuploidy regions and on the 4th chromosome this compensation is mediated by POF (Painting of Fourth).

Keywords: dose response
 
Overall design We prepared total RNA from flies heterozygous for three different deletions, Df(2L)J-H, Df (2L)ED4470, Df(2L)ED4651, flies heterozygous for the duplication Dp(2;2)Cam3 and flies with only one chromosome 4, or three copies of the 4th chromosome, as well as from wild type control flies. Three biological replicates of all genotypes were prepared.
 
Contributor(s) Stenberg P, Larsson J
Citation(s) 19412336
Submission date Jan 22, 2009
Last update date Aug 28, 2018
Contact name Jan Larsson
E-mail(s) jan.larsson@molbiol.umu.se
Organization name Umea University
Department Molecular Biology
Lab Jan Larsson
Street address Umea University
City Umea
ZIP/Postal code SE-90187
Country Sweden
 
Platforms (1)
GPL1322 [Drosophila_2] Affymetrix Drosophila Genome 2.0 Array
Samples (27)
GSM362897 Adult_female_pof-mutant_biological_replicate_1
GSM362898 Adult_female_pof-mutant_biological_replicate_2
GSM362899 Adult_female_pof-mutant_biological_replicate_3
Relations
BioProject PRJNA111665

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE14517_RAW.tar 55.1 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table
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