|
Status |
Public on Nov 16, 2020 |
Title |
Next Generation Sequencing Facilitates Quantitative Analysis of chromatin interaction of lncRNA Gm4665 with regulatory region of target genes in male germ cells. |
Organism |
Mus musculus |
Experiment type |
Other
|
Summary |
Purpose: The goals of this study are to examine the possibility that Gm4665 binds to regulatory region of target genes loci in male germ cells. Methods: we performed the Chromatin Isolation by RNA Purification (ChIRP) assay to examine the possibility that Gm4665 binds to regulatory region of target genes. Germ cell isolated from the testis of wild-type mouse were cross-linked, lysed and sonicated, and then incubated with probesets of Gm4665. Following hybridization of biotinylated probesets to Gm4665 transcript, Gm4665-bound chromatin was retrieved. The purified DNA fragments were sequenced using high throughput next generation sequencing (Illumina HiseqXten-PE150) Results: Analysis of the retrieved chromatin fragments by deep sequencing (ChIRP-seq) revealed Gm4665 occupancy sites were genome wide, specifically abundant enrich in intronic regions. Additionally, nearly 30% of chromatin fragments were enriched in the promoter regions of gene loci
|
|
|
Overall design |
Examination of chromatin interaction of lncRNA Gm4665 with regulatory region of target genes in male germ cells
|
|
|
Citation missing |
Has this study been published? Please login to update or notify GEO. |
Submission date |
Feb 12, 2020 |
Last update date |
Nov 16, 2020 |
Contact name |
Xu jia yue |
E-mail(s) |
xujiayue78@126.com
|
Organization name |
Peking Union Medical College
|
Street address |
NO.3 Dongdan street, Beijing
|
City |
Beijing |
ZIP/Postal code |
100010 |
Country |
China |
|
|
Platforms (1) |
|
Samples (9)
|
|
Relations |
BioProject |
PRJNA606277 |
SRA |
SRP248451 |