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| Status |
Public on Feb 19, 2020 |
| Title |
Expression data from INS1E cells stimulated with vitamin D metabolites and glucose |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by array
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| Summary |
Studies have shown that vitamin D can enhance glucose-stimulated insulin secretion (GSIS) and change the expression of genes in pancreatic β-cells. Still the mechanisms linking vitamin D and GSIS are unknown.
We used an established β-cell line, INS1E. INS1E cells were pre-treated with 10 nM 1,25(OH)2vitamin D or 10 nM 25(OH)vitamin D for 72 hours and stimulated with 22 mM glucose for 60 minutes. RNA was extracted for gene expression analysis.
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| Overall design |
After four passages INS1E cells (100 000 cells per well) were seeded in RPMI with 10 nM 25(OH)vitamin D (Sigma-Aldrich, Netherlands), RPMI with 10 nM 1,25(OH)2vitamin D (Sigma-Aldrich, Israel) or RPMI with standard supplements alone (control cells). After 72 hours of preincubation, glucose stimulation was carried out. The cells were first incubated with glucose-free Krebs-Ringer buffer for 60 minutes, and immediately after they were subjected to stimulation with 22 mM glucose in Krebs-Ringer buffer in triplicates for 60 minutes.
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| Contributor(s) |
Bornstedt ME, Olstad OK |
| Citation(s) |
32251702 |
| Submission date |
Feb 18, 2020 |
| Last update date |
Apr 08, 2020 |
| Contact name |
Mette Eskild Bornstedt |
| E-mail(s) |
metbo@icloud.com, metbo@ous-hf.no
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| Phone |
0047 91822819
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| Organization name |
Oslo University Hospital
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| Department |
Dep of Medical Biochemistry
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| Lab |
Hormone laboratory
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| Street address |
Pb 4959 Nydalen
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| City |
Oslo |
| ZIP/Postal code |
N-0424 |
| Country |
Norway |
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| Platforms (1) |
| GPL6247 |
[RaGene-1_0-st] Affymetrix Rat Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (18)
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| Relations |
| BioProject |
PRJNA607263 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE145460_Microarray_INS1E_Normalized_Signal_Values_Mette_Eskild_Bornstedt.xlsx |
4.3 Mb |
(ftp)(http) |
XLSX |
| GSE145460_RAW.tar |
70.4 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
| Processed data are available on Series record |
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