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Series GSE145523 Query DataSets for GSE145523
Status Public on Jul 04, 2020
Title Triacylglycerol synthesis enhances macrophage inflammatory function
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Macrophages stimulated with lipopolysaccharide (LPS) plus interferon-g (IFNg) accumulate TGs in LDs, and long-chain acylcarnitines. Inhibition of TG synthesis results in diminished LD development, and increased long chain acylcarnitine levels, suggesting that FA fate is balanced between TG and acylcarnitine synthesis. Nevertheless, TG-synthesis is required for inflammatory macrophage function, since its inhibition negatively affects production of proinflammatory IL-1b, IL-6 and PGE2, and phagocytic capacity, and protects against LPS-induced sock in vivo.
 
Overall design RNA-Seq was performed on resting or activated Macrophages treated with or without the DGAT1 inhibitor T863
 
Contributor(s) Castoldi A, Monteiro LB, van Teijlingen Bakker N, Sanin DE, Rana N, Corrado M, Cameron AM, Hässler F, Matsushita M, Caputa G, Klein Geltink RI, Büscher J, Edwards-Hicks J, Pearce EL, Pearce EJ
Citation(s) 32796836
Submission date Feb 19, 2020
Last update date Aug 31, 2020
Contact name Immunometabolism Department
E-mail(s) dsaninp1@jhmi.edu
Organization name Johns Hopkins University
Department Immunometabolism
Street address 1650 Orleans Street
City Baltimore
State/province Maryland
ZIP/Postal code 21287
Country USA
 
Platforms (1)
GPL21493 Illumina HiSeq 3000 (Mus musculus)
Samples (12)
GSM4320173 Resting_M0_1
GSM4320174 Resting_M0_2
GSM4320175 Resting_M0_3
Relations
BioProject PRJNA607504
SRA SRP249995

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE145523_Raw_Read_Count_Matrix.txt.gz 455.6 Kb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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