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| Status |
Public on Jul 04, 2020 |
| Title |
Triacylglycerol synthesis enhances macrophage inflammatory function |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Macrophages stimulated with lipopolysaccharide (LPS) plus interferon-g (IFNg) accumulate TGs in LDs, and long-chain acylcarnitines. Inhibition of TG synthesis results in diminished LD development, and increased long chain acylcarnitine levels, suggesting that FA fate is balanced between TG and acylcarnitine synthesis. Nevertheless, TG-synthesis is required for inflammatory macrophage function, since its inhibition negatively affects production of proinflammatory IL-1b, IL-6 and PGE2, and phagocytic capacity, and protects against LPS-induced sock in vivo.
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| Overall design |
RNA-Seq was performed on resting or activated Macrophages treated with or without the DGAT1 inhibitor T863
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| Contributor(s) |
Castoldi A, Monteiro LB, van Teijlingen Bakker N, Sanin DE, Rana N, Corrado M, Cameron AM, Hässler F, Matsushita M, Caputa G, Klein Geltink RI, Büscher J, Edwards-Hicks J, Pearce EL, Pearce EJ |
| Citation(s) |
32796836 |
| Submission date |
Feb 19, 2020 |
| Last update date |
Aug 31, 2020 |
| Contact name |
Immunometabolism Department |
| E-mail(s) |
dsaninp1@jhmi.edu
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| Organization name |
Johns Hopkins University
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| Department |
Immunometabolism
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| Street address |
1650 Orleans Street
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| City |
Baltimore |
| State/province |
Maryland |
| ZIP/Postal code |
21287 |
| Country |
USA |
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| Platforms (1) |
| GPL21493 |
Illumina HiSeq 3000 (Mus musculus) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA607504 |
| SRA |
SRP249995 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE145523_Raw_Read_Count_Matrix.txt.gz |
455.6 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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