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| Status |
Public on Dec 01, 2004 |
| Title |
CxVB3 infection and cardiac function |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Male, adolescent A/J mice (4-5 week old) were either infected (IP) with coxsackievirus B3 (CVB3; 105 pfu) or PBS. CVB3 is a cardiotropic virus which leads to cardiac inflammation and fibrosis within 9 days after IP injection. We wanted to know how the virus would impact long term cardiac function, and whether changes in cardiac function could be associated with cardiac transcriptional regulation. At days 3, 9 and 30 days post-infection, hearts were imaged with 2D echocardiography (Sonos 5500, Philips). Briefly, mice were anesthesized subcutaneously with ketamine (0.45mg/kg) and xylazine (0.03mg/kg). Mice were placed in a dorsal recumbency position and the following systolic and diastolic measurements were taken (N=5 heart beats) using an S-12 (12MHz; Sonos 5000, Philips) probe: parasternal long axis, and short axis at the level of the mitral valve and papillary muscles. Measurements were utilized for calculation of wall thickness and functional parameters. Then, heart tissues were flash frozen (N=4 mice/group except CVB3 infected 30 day samples N=2) for hybridization to Affymetrix MG U74Av2 arrays.
Keywords: time-course
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| Contributor(s) |
Yanagawa B, Luo H, Deisher TA, Cheung C, Yuan J, Hollander Z, Wong B, Kapounx AM, Bonigut S, Yang D, Schreinerx GF, Ng R, McManus BM |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Jun 04, 2004 |
| Last update date |
Feb 18, 2018 |
| Contact name |
John Gosink |
| E-mail(s) |
gosinkj@amgen.com
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| Phone |
206 265-8217
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| Organization name |
Amgen
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| Street address |
1201 Amgen Court West
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| City |
Seattle |
| State/province |
WA |
| ZIP/Postal code |
98119 |
| Country |
USA |
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| Platforms (1) |
| GPL81 |
[MG_U74Av2] Affymetrix Murine Genome U74A Version 2 Array |
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| Samples (7)
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| GSM24472 |
pooled day 30 hearts from PBS injected mice |
| GSM24473 |
5 pooled baseline hearts |
| GSM24474 |
pooled day 3 hearts from PBS injected mice |
| GSM24475 |
pooled day 3 hearts from CxVB3 injected mice |
| GSM24476 |
pooled day 9 hearts from PBS injected mice |
| GSM24477 |
pooled day 9 hearts from CxVB3 injected mice |
| GSM24478 |
pooled day 30 hearts from CxVB3 injected mice |
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| Relations |
| BioProject |
PRJNA90009 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE1457_RAW.tar |
22.3 Mb |
(http)(custom) |
TAR (of CEL) |
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