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| Status |
Public on Jul 29, 2020 |
| Title |
Robust intrathymic development of regulatory T cells in young NOD mice is rapidly restrained by recirculating cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Regulatory T lymphocytes (Treg) play a vital role in the protection of the organism against autoimmune pathology. It is therefore paradoxical that comparatively large numbers of Treg were found in the thymus of type I diabetes‐prone NOD mice. The Treg population in the thymus is composed of newly developing cells and cells that had recirculated from the periphery back to the thymus. We here demonstrate that exceptionally large numbers of Treg develop in the thymus of young, but not adult, NOD mice. Once emigrated from the thymus, an unusually large proportion of these Treg is activated in the periphery, which causes a particularly abundant accumulation of recirculating Treg in the thymus. These cells then rapidly inhibit de novo development of Treg. The proportions of developing Treg thus reach levels similar to or lower than those found in most other, type 1 diabetes‐resistant, inbred mouse strains. Thus, in adult NOD mice the particularly large Treg‐niche is actually composed of mostly recirculating cells and only few newly developing Treg.
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| Overall design |
Here we show the gene expression by RNAseq from regulatory T cells recirculating to the thymus, in NOD and B6, 3 weeks old mouse strains.
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| Contributor(s) |
Darrigues J, Santamaria JC, Galindo-Albarrán AO, Robey EA, Joffre OP, van Meerwijk JM, Romagnoli P |
| Citation(s) |
32730634 |
| Submission date |
Feb 21, 2020 |
| Last update date |
Oct 28, 2020 |
| Contact name |
Joost van Meerwijk |
| E-mail(s) |
Joost.van-Meerwijk@inserm.fr
|
| Phone |
0562748381
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| Organization name |
INSERM
|
| Department |
U1043
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| Lab |
Joost van Meerwijk Eq1
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| Street address |
CHU Purpan – BP 3028
|
| City |
Toulouse |
| State/province |
HG |
| ZIP/Postal code |
31024 |
| Country |
France |
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| Platforms (1) |
| GPL21493 |
Illumina HiSeq 3000 (Mus musculus) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA607982 |
| SRA |
SRP250295 |
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