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Status |
Public on Jul 16, 2021 |
Title |
DNA hyper-methylation during Tuberculosis dampens host immune responsiveness |
Organism |
Homo sapiens |
Experiment type |
Methylation profiling by genome tiling array
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Summary |
DNA Methylation was analyzed in tuberculosis patients without HIV at baseline and 6 months after completion of successful therapy. DNA methylation was also evaluated in TB patients with HIV and in healthy controls without Tuberculosis. 500ng of gDNA was bisulfite treated prior to running the Illumina Infinium MethylEPIC array
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Overall design |
The study protocol was reviewed by the Baylor College of Medicine Children’s Foundation-Swaziland, Baylor College of Medicine and Eswatini National Health Research and Review Board. All participation was voluntary and implemented in accord with the institutional and international guidelines for the Protection of Human Subjects in concordance with the Declaration of Helsinki including written informed consent from participants or their guardians. In this analysis, immune cells of TB participants were analyzed if they had pulmonary disease as defined by symptoms of TB (cough, fevers, weight loss, etc) and microbiologically confirmed disease by sputum Gene Xpert and/ or culture. All participants were screened for HIV. Healthy controls were household contacts of TB patients with a TB contact score of five or greater and excluded if they progressed to active TB and did not remain asymptomatic for 12- months. All individuals were BCG vaccinated as confirmed by BCG scar and/ or vaccine card.
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Contributor(s) |
DiNardo AR, Rajapakshe K, Nishiguchi T, Grimm SL, Mtetwa G, Dlamini Q, Kahari J, Mahapatra S, Kay A, Maphalala G, Mace EM, Makedonas G, Cirillo JD, Netea MG, van Crevel R, Coarfa C, Mandalakas AM |
Citation(s) |
32125282 |
Submission date |
Feb 21, 2020 |
Last update date |
Jul 17, 2021 |
Contact name |
Andrew R DiNardo |
E-mail(s) |
Andrew.Dinardo@bcm.edu
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Phone |
2152064388
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Organization name |
BCM
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Department |
Global and Immigrant Health
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Street address |
1102 Bates St
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City |
Houston |
State/province |
Texas |
ZIP/Postal code |
77030 |
Country |
USA |
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Platforms (1) |
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Samples (32)
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Relations |
BioProject |
PRJNA607988 |
Supplementary file |
Size |
Download |
File type/resource |
GSE145714_Matrix_signal_intensities.txt.gz |
138.0 Mb |
(ftp)(http) |
TXT |
GSE145714_RAW.tar |
612.7 Mb |
(http)(custom) |
TAR (of IDAT) |
Processed data included within Sample table |
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