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| Status |
Public on Mar 22, 2021 |
| Title |
Transcriptome analysis of Bcl11b-deficient MAIT cells at steady state and infection |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Mucosal associated invariant T (MAIT) cells, already differentiated and located at mucosal sites, are critical in the body’s first wave of defenses against invading pathogens. Bcl11b KO MAIT cells fail to be maintained both in the thymus and peripheral organs. Furthermore, MAIT cells fail to fully develop in the thymus without Bcl11b, failing to upregulate RORγt, and that phenotype remains in the lungs and livers of these mice. Bcl11b deletion in MAIT cells causes dramatic shifts in the activation and TH17 programs, due to the binding of Bcl11b in many of those genes, which we have seen in the human MAIT cells. MAIT cells rely on PLZF and RORγt for their development and function, while also heavily relying on Bcl11b. These data show the key interplay of Bcl11b with PLZF and RORγt in a T cell leading to its development and necessary function to protect the body against diseases.
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| |
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| Overall design |
Examination of changes to transcriptome resulting from removal of Bcl11b in MAIT cells
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| |
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| Contributor(s) |
Drashansky T, Helm E, Huo Z, Avram D |
| Citation(s) |
33870128 |
| Submission date |
Feb 23, 2020 |
| Last update date |
May 25, 2021 |
| Contact name |
Dorina Avram |
| E-mail(s) |
dorina.avram@moffitt.org
|
| Organization name |
Moffitt Cancer Center
|
| Department |
Department of Immunology
|
| Lab |
Avram lab
|
| Street address |
12902 Magnolia Drive, MRC4
|
| City |
Tampa |
| State/province |
Fl |
| ZIP/Postal code |
33612 |
| Country |
USA |
| |
|
| Platforms (2) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
| GPL21493 |
Illumina HiSeq 3000 (Mus musculus) |
|
| Samples (8)
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| This SubSeries is part of SuperSeries: |
| GSE145779 |
Bcl11b binding and transcriptome analysis of Bcl11b-deficient MAIT cells at steady state and infection |
|
| Relations |
| BioProject |
PRJNA608200 |
| SRA |
SRP250435 |
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