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| Status |
Public on Feb 26, 2020 |
| Title |
Conversion of fibroblasts to functional Leydig like cells using defined small molecules |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
we report that fibroblasts can be efficiently and directly reprogrammed into Leydig-like cells by exposing to a combination of small molecules, Forskolin, 20α-hydroxycholesterol, luteinizing hormone (LH), and SB431542. The chemical compounds induced Leydig-like cells (CiLCs) expressed steroidogenic genes, had a global gene expression profile similar to that of Leydig cell, and acquired androgen synthesis capabilities.
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| Overall design |
A total of 6 samples（2 CiLCs, 2 MEFs, 2 LCs） were tested using the BGISEQ platform for this project to prove that the CiLC resemble Leydig cell in genome-wide gene expression profile.
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| Contributor(s) |
Yang Y, Zhou C |
| Citation(s) |
32735821 |
| Submission date |
Feb 24, 2020 |
| Last update date |
Aug 24, 2020 |
| Contact name |
Chengxing Zhou |
| E-mail(s) |
zhou.chengxing@outlook.com
|
| Organization name |
Jinan University
|
| Street address |
Jinan University
|
| City |
Guangzhou |
| ZIP/Postal code |
510632 |
| Country |
China |
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| Platforms (1) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA608265 |
| SRA |
SRP250472 |
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