|
| Status |
Public on Nov 05, 2020 |
| Title |
Metabolic Effects of Air Pollution Exposure and Reversibility |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
In this study, we modeled early life air pollution exposure using C57BL/6J male mice on a controlled chow diet, exposed to real-world inhaled concentrated PM2.5 (~10x ambient level/ ~60-120g/m3) or filtered air (FA) over 14 weeks. We investigated PM2.5 effects on phenotype, transcriptome and chromatin accessibility, compared the effects with a prototypical high-fat diet (HFD) stimulus, and examined the effects of cessation of exposure on reversibility of phenotype/genotype.
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| |
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| Overall design |
RNA sequencing and ATAC-seq were performed to compare the transcriptome and epigenome of air pollution exposed mice and high-fat diet.
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| |
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| Contributor(s) |
Rajagopalan S, Park B, Hansen K, Biswal S |
| Citation(s) |
32780721 |
| Submission date |
Feb 24, 2020 |
| Last update date |
Nov 05, 2020 |
| Contact name |
Bongsoo Park |
| E-mail(s) |
bongsoo.park@nih.gov
|
| Phone |
1-410-558-8510
|
| Organization name |
National Institute on Aging
|
| Department |
Translational Gerontology Branch
|
| Lab |
Epigenetics and Stem Cell Aging
|
| Street address |
251 Bayview Blvd
|
| City |
Baltimore |
| State/province |
Maryland |
| ZIP/Postal code |
21224 |
| Country |
USA |
| |
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| Platforms (1) |
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| Samples (94)
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| Relations |
| BioProject |
PRJNA608484 |
| SRA |
SRP250559 |
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