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Status |
Public on Feb 16, 2021 |
Title |
H3K27ac ChIP-seq for multiple myeloma cell lines, B-lymphoma cells and normal plasma cell |
Organism |
Homo sapiens |
Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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Summary |
ChIP-seq analysis was performed in Multiple Myeloma cell lines to analyze acetylation of histone H3K27ac.
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Overall design |
Daudi, RAJI, H929, JJN3, KMS11, KMS12, KMS28BM, RPMI8226, U266 and NP (normal plasma cell) were crosslinked with formaldehyde for 10 min. DNA was enriched by chromatin-immunoprecipitation (ChIP) and analyzed by Illumina Hiseq 4000. A sample of whole cell extract (WCE) was sequenced and used as the background to determine enrichment. ChIP was performed using an antibody against histone H3 lysine 27 acetylation (H3K27Ac: Abcam, ab4729).
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Contributor(s) |
Jia Y, Tan TK, Joo CW |
Citation(s) |
33579893 |
Submission date |
Feb 25, 2020 |
Last update date |
Feb 16, 2021 |
Contact name |
Tze King Tan |
Organization name |
National University of Singapore
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Department |
Cancer Science Institute
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Lab |
Takaomi Sanda Lab
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Street address |
14 Medical Drive
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City |
Singapore |
ZIP/Postal code |
117599 |
Country |
Singapore |
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Platforms (1) |
GPL20301 |
Illumina HiSeq 4000 (Homo sapiens) |
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Samples (20)
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This SubSeries is part of SuperSeries: |
GSE145938 |
Super-enhancer profiling combined with CDK7 inhibition-transcripts identifies novel oncogenes of multiple myeloma. |
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Relations |
BioProject |
PRJNA608682 |
SRA |
SRP250673 |