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Series GSE145890 Query DataSets for GSE145890
Status Public on Feb 16, 2021
Title H3K27ac ChIP-seq for multiple myeloma cell lines, B-lymphoma cells and normal plasma cell
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary ChIP-seq analysis was performed in Multiple Myeloma cell lines to analyze acetylation of histone H3K27ac.
 
Overall design Daudi, RAJI, H929, JJN3, KMS11, KMS12, KMS28BM, RPMI8226, U266 and NP (normal plasma cell) were crosslinked with formaldehyde for 10 min. DNA was enriched by chromatin-immunoprecipitation (ChIP) and analyzed by Illumina Hiseq 4000. A sample of whole cell extract (WCE) was sequenced and used as the background to determine enrichment. ChIP was performed using an antibody against histone H3 lysine 27 acetylation (H3K27Ac: Abcam, ab4729).
 
Contributor(s) Jia Y, Tan TK, Joo CW
Citation(s) 33579893
Submission date Feb 25, 2020
Last update date Feb 16, 2021
Contact name Tze King Tan
Organization name National University of Singapore
Department Cancer Science Institute
Lab Takaomi Sanda Lab
Street address 14 Medical Drive
City Singapore
ZIP/Postal code 117599
Country Singapore
 
Platforms (1)
GPL20301 Illumina HiSeq 4000 (Homo sapiens)
Samples (20)
GSM4338484 Daudi_H3K27ac_ChIP-Seq
GSM4338485 Daudi_ChIP_Input
GSM4338486 H929_H3K27ac_ChIP-Seq
This SubSeries is part of SuperSeries:
GSE145938 Super-enhancer profiling combined with CDK7 inhibition-transcripts identifies novel oncogenes of multiple myeloma.
Relations
BioProject PRJNA608682
SRA SRP250673

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE145890_RAW.tar 10.9 Mb (http)(custom) TAR (of BROADPEAK)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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