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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Dec 31, 2009 |
| Title |
Virally infected LSEC trigger CD8 T cell immunity |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Dendritic cell activation through ligation of pattern recognition receptors leading to full functional maturation causes induction of CD8 T cell immunity instead of tolerance through increased delivery of co-stimulatory signals. Here we investigate whether a prototypic organ-resident antigen-presenting cells, i.e. liver sinusoidal endothelial cells (LSEC), also switch from tolerogenic to immunogenic CD8 T cell activation upon such activation. We demonstrate by gene expression analysis that LSEC expressed numerous pattern recognition receptors that attributed sentinel function, but ligand-induced activation of these receptors was not sufficient to overcome tolerance induction of CD8 T cells. However, viral infection caused functional maturation of antigen-presenting LSEC and was sufficient to promote antigen-specific differentiation into fully functional effector CD8 T cells in the absence of dendritic cells. Thus, we identify a novel principle of inducing CD8 T cell immunity by virally infected organ-resident antigen-presenting cells employing distinct mechanisms compared to dendritic cells.
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| Overall design |
Transcriptional profiles of untreated liver sinusoidal endothelial cells (LSEC) and LSEC, stimulated with either LPS (100ng/ml) or infected with MCMV-GFP (MOI=0.1) were compared on Illumina Mouse WG-6 BeadChip microarray platform. All samples represent biological replicates and were processed separately throughout the experiment.
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| Contributor(s) |
Kern M, Popov A, Schultze JL, Knolle PA |
| Citation(s) |
19737567 |
| Submission date |
Feb 02, 2009 |
| Last update date |
Mar 20, 2012 |
| Contact name |
Michaela Kern |
| E-mail(s) |
Michaela.kern@ukb.uni-bonn.de
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| Organization name |
University of Bonn
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| Street address |
Sigmund-Freud-Str. 25
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| City |
Bonn |
| ZIP/Postal code |
53105 |
| Country |
Germany |
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| Platforms (1) |
| GPL4234 |
Sentrix Mouse-6 Expression BeadChip |
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| Samples (18)
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| GSM366239 |
Liver sinusoidal endothelial cells, sample 1 |
| GSM366243 |
Liver sinusoidal endothelial cells, treated with LPS, sample 1 |
| GSM366244 |
Liver sinusoidal endothelial cells, infected with MCMV, sample 1 |
| GSM369358 |
Liver sinusoidal endothelial cells, sample 2 |
| GSM369990 |
Liver sinusoidal endothelial cells, sample 3 |
| GSM369991 |
Liver sinusoidal endothelial cells, sample 4 |
| GSM369992 |
Liver sinusoidal endothelial cells, sample 5 |
| GSM369993 |
Liver sinusoidal endothelial cells, sample 6 |
| GSM369994 |
Liver sinusoidal endothelial cells, treated with LPS, sample 2 |
| GSM369995 |
Liver sinusoidal endothelial cells, treated with LPS, sample 4 |
| GSM370086 |
Liver sinusoidal endothelial cells, treated with LPS, sample 3 |
| GSM370087 |
Liver sinusoidal endothelial cells, infected with MCMV, sample 2 |
| GSM370088 |
Liver sinusoidal endothelial cells, infected with MCMV, sample 3 |
| GSM370089 |
Liver sinusoidal endothelial cells, infected with MCMV, sample 4 |
| GSM370090 |
Liver sinusoidal endothelial cells, infected with MCMV, sample 5 |
| GSM370091 |
Liver sinusoidal endothelial cells, infected with MCMV, sample 6 |
| GSM370092 |
Liver sinusoidal endothelial cells, infected with MCMV, sample 7 |
| GSM370093 |
Liver sinusoidal endothelial cells, infected with MCMV, sample 8 |
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| Relations |
| BioProject |
PRJNA112373 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE14674_RAW.tar |
5.4 Mb |
(http)(custom) |
TAR (of TXT) |
| Processed data included within Sample table |
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