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| Status |
Public on Sep 13, 2020 |
| Title |
mTORC1 and B cell survival signal regulate formation of memory precursors in the germinal center [1] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
A still unanswered question is what drives the small fraction of activated germinal center (GC) B cells that survive to become long-lived quiescent memory B cells. We found here that a small population of light zone GC cells (CD38intBcl6hi/intEfnb1+) with lower mTORC1 activity favored the memory B cell fate. Constitutively high mTORC1 activity led to defects in formation of the CD38intBcl6hi/intEfnb1+ cells; conversely, decreasing mTORC1 activity resulted in relative enrichment of this memory-prone GC population over the recycling-prone one. Furthermore, the CD38intBcl6hi/intEfnb1+ cells had higher levels of Bcl2 and surface BCR, which in turn, contributed to their survival. Given the positive correlation between the strength of T cell help and mTORC1 activity, our data suggest a model in which weak help from T cells together with provision of an increased survival signal are key for GC B cells to assume a memory B cell fate.
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| Overall design |
mRNA profiles of Blimp1 single deficient, and Bach2/Blimp1 double deficient DZ, LZ GC B cells were generated by deep sequencing in duplicate, using Illumina HiSeq 1500.
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| Contributor(s) |
Inoue T, Kawai C, Ohara O, Kurosaki T |
| Citation(s) |
33045065 |
| Submission date |
Mar 13, 2020 |
| Last update date |
Nov 04, 2020 |
| Contact name |
Takeshi Inoue |
| Organization name |
Osaka University
|
| Street address |
3-1 Yamadaoka
|
| City |
Suita-shi |
| State/province |
Osaka |
| ZIP/Postal code |
5650871 |
| Country |
Japan |
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| Platforms (1) |
| GPL18480 |
Illumina HiSeq 1500 (Mus musculus) |
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| Samples (8)
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| This SubSeries is part of SuperSeries: |
| GSE147096 |
mTORC1 and B cell survival signal regulate formation of memory precursors in the germinal center |
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| Relations |
| BioProject |
PRJNA612434 |
| SRA |
SRP252680 |
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