NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Series GSE146985 Query DataSets for GSE146985
Status Public on Aug 26, 2021
Title Characterization of pancreatic progenitor differentiation with GiBi-generated DE cells
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary We report the GiBi protocol to generate functional DE cells that can further enter pancreatic lineages. To understand the transcriptome dynamics during the generation of these pancreatic progenitor cells, we harvested cells at different time points of the differentiation for RNA sequencing. We find that during this process, genes that are related to pluripotency maintenance were decreasing and gastrulation-related genes were transiently expressed in the middle. More importantly, genes that are critical in pancreatic specification got significantly upregulated across the differentiation.
 
Overall design Every other day from D0 to D14 of the pancreatic progenitor differentiation, two biological replicates for each time point, we harvested in total 14 samples for RNA sequencing.
 
Contributor(s) Jiang Y, Lian X
Citation(s) 34450037
Submission date Mar 14, 2020
Last update date Oct 06, 2021
Contact name Yuqian Jiang
E-mail(s) yuqianjiang2016@gmail.com
Organization name PSU
Street address W345 millennium science complex
City University park
State/province PA
ZIP/Postal code 16801
Country USA
 
Platforms (1)
GPL21697 NextSeq 550 (Homo sapiens)
Samples (14)
GSM4411759 H1_PP_D0_R1
GSM4411760 H1_PP_D0_R2
GSM4411761 H1_PP_D2_R1
Relations
BioProject PRJNA612623
SRA SRP252813

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE146985_RAW.tar 33.6 Mb (http)(custom) TAR (of TSV)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap