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| Status |
Public on Aug 24, 2020 |
| Title |
Role of TGF-β signaling in hypoxia-induced alternative splicing in breast cancer cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Analysis of transcriptome post hypoxia and TGF-β treatment in breast cancer
In order to explore the role of TGF-β signaling in mediating the alternative splicing program in breast cancer, we profiled the pre-mRNA splicing and mRNA gene expression upon hypoxia and TGF-β treatment using Human Transcriptome Array 2.0.
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| Overall design |
Breast cancer cell lines MCF7 and HCC1806 were cultured in 1% O2 for giving hypoxia treatment. For TGF-β induction, cells were treated with 10 ng/ml of TGF-β ligand. Post harvest, samples were analysed on Human Transcriptome Array 2.0 platform. Raw files generated were processed using Transcriptome Analysis Console tool.
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| Contributor(s) |
Ahuja N, Ashok C, Natua S, Pant D, Cherian A, Pandkar MR, Yadav P, Narayanan VS, Mishra J, Samaiya A, Shukla S |
| Citation(s) |
33089214 |
| Submission date |
Mar 25, 2020 |
| Last update date |
Oct 27, 2020 |
| Contact name |
Sanjeev Shukla |
| E-mail(s) |
sanjeevs@iiserb.ac.in
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| Phone |
9584635471
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| Organization name |
Indian Institute of Science Education and Research Bhopal
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| Department |
Biological Sciences
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| Lab |
Epigenetics and RNA Processing Lab (ERPL)
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| Street address |
Bhopal Bypass Road, Bhauri Bhopal 462 066 Madhya Pradesh, INDIA
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| City |
Bhopal |
| State/province |
Madhya Prades |
| ZIP/Postal code |
462066 |
| Country |
India |
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| Platforms (1) |
| GPL17586 |
[HTA-2_0] Affymetrix Human Transcriptome Array 2.0 [transcript (gene) version] |
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| Samples (16)
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| Relations |
| BioProject |
PRJNA615099 |
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