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Series GSE14763 Query DataSets for GSE14763
Status Public on Feb 09, 2010
Title Whole transcriptome analysis of the hippocampus: toward a molecular portrait of epileptogenesis
Organism Rattus norvegicus
Experiment type Expression profiling by array
Summary Purpose: to evaluate changes in the transcriptome of hippocampal cells during the course of epileptogenesis, from the early events post status epilepticus (SE) to the onset of recurrent spontaneous seizures.
Experimental Design: Gene expression profiling was analyzed in hippocampi of rats subjected to the pilocarpine model of epilepsy at different times during the course of epileptogenesis: 3 days post-SE (3D), 7 days post-SE (7D), and immediately (up to 12h) after the first spontaneous seizure (Chronic). All three pilocarpine subgroups had a corresponding age-matched control group (saline-treated rats), from which normal hippocampi samples were obtained at the same experimental time points. Independent microarray hybridizations were carried out for each sample (n = 5, per experimental group) with oligonucleotide microarrays covering 34,000 transcripts representing most of the known and predictive genes of the rat genome (CodeLink™ Rat Whole Genome Bioarrays, GE Healthcare), following the manufacturer’s protocol.
Results: differential expression of almost 1,400 genes was detected during the course of epileptogenesis, from the early events post status epilepticus (SE) to the onset of recurrent spontaneous seizures. Most of these genes are novel and displayed an up-regulation after SE. Noteworthy, a group of 128 genes functioning in neurogenesis, apoptosis, immune response, and intracellular signal transduction was found consistently hyper-expressed throughout epileptogenesis, indicating stable molecular alterations within the hippocampus. Those include modulation of the MAPK, Jak-STAT, PI3K, TGF-beta, and mTOR signaling pathways. Differential expression of genes from the p38 MAPK pathway, mediating inflammation and neurogenesis, was also confirmed by real-time PCR. These findings reveal dynamic molecular changes occurring in the hippocampus that may serve as a starting point for the generation of new hypothesis regarding the underlying mechanisms of epilepsy and for the designing of alternative therapeutic strategies.
Keywords: gene expression changes during epileptogenesis.
Overall design We performed a genome wide analysis of genes differentially expressed during epileptogenesis. Virtually, all possible changes in the rat transcriptome were monitored at distinct time points corresponding to the latent to chronic phase transition of the pilocarpine model of epilepsy, which is probably the most extensively studied chemically inductive model of TLE. Genes identified as being differentially expressed were classified based on their respective biological functions to envisage processes and pathways likely implicated in epileptogenesis, as well as their possible value as targets for therapy.
Results were expressed as fold variation, and genes displaying greater than 2-fold changes in transcript abundance and p<0.01 were selected.
Contributor(s) Okamoto OK, Janjoppi L, Bonone FM, Pansani AP, Silva AV, Scorza FA, Cavalheiro EA
Citation(s) 20377889
Submission date Feb 09, 2009
Last update date Jun 06, 2014
Contact name Oswaldo Keith Okamoto
Organization name UNIFESP
Department Neurology
Lab Experimental Neurology
Street address R. Botucatu, 862.
City Sao Paulo
State/province SP
ZIP/Postal code 04023-900
Country Brazil
Platforms (1)
GPL2896 GE Healthcare/Amersham Biosciences CodeLink™ Rat Whole Genome Bioarray
Samples (18)
GSM367527 hippocampus_12_3H
GSM367528 hippocampus_11_3H
GSM367531 hippocampus_13_3H
BioProject PRJNA112259

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE14763_RAW.tar 23.9 Mb (http)(custom) TAR (of TXT)
Raw data provided as supplementary file
Processed data included within Sample table

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