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| Status |
Public on Oct 20, 2021 |
| Title |
Next Generation Sequencing Facilitates Quantitative Analysis of Transcriptome profile in IEC 4.1-WT and IEC4.1-Ifnar1-/- KO cells (C12 KO) in response to Cryptosporidium parvum |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
This study aims to measure the impact of Ifnar1-/- knockdown (KO) on the transcriptome profile in cultured IEC 4.1 cell in response to C. parvum infection. IEC4.1-WT cells and IEC4.1-Ifnar1-/- cells (stably CRISPR/Cas9 knockout; C12 KO cells) were exposed to C. parvum infection. Cells treated with a scrambled non-specific siRNA were used as the control. Total RNA was collected for the genome-wide analysis.
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| Overall design |
The murine intestinal epithelial cells were grown to 80% confluence for four groups: IEC4.1 control (Group A, A1-A3), IEC4.1 + C. parvum 24h (Group E, E1-E3, IEC 4.1 cells exposed to C. parvum infection for 24h), C12 control (Group C, C1-C3), C12 + C. parvum 24h (Group F, F1-F3, C12 cells exposed to C. parvum infection for 24h). After treatment, total RNAs were prepared with the RNeasy Mini kit (Qiagen) according to the manufacturer’s instruction.
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| Contributor(s) |
Chen X |
| Citation(s) |
36928642 |
| Submission date |
Mar 30, 2020 |
| Last update date |
Jul 14, 2023 |
| Contact name |
Xianming Chen |
| Organization name |
creighton university
|
| Street address |
2500 california plaza
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| City |
Omaha |
| State/province |
NE |
| ZIP/Postal code |
68178 |
| Country |
USA |
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| Platforms (1) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA616254 |
| SRA |
SRP254538 |
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