|
| Status |
Public on Dec 09, 2020 |
| Title |
To study the function of KLF5 in squamous cancers and gastrointestinal cancers [ChIP-seq] |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
To identify KLF5 target genes and study the gene regulation function of KLF5 by integrating ChIP-seq, HiChIP and RNA-seq assays
|
| |
|
| Overall design |
ChIP-seq to identify KLF5 binding site in squamous cancer and gastrointestinal cell lines; ChIP-seq to define BRD4 and phosphorylated POL2 binding profiles in HARA cells with and without KLF5 knockout. Samples with 'sgAAVS1' use a sgRNA targeting an intronic region of AAVS1, which serves a negative control for CRISPR knockout.
|
| |
|
| Contributor(s) |
Zhang X, Liu Y |
| Citation(s) |
33115806 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| R00 CA215244 |
Investigating the role of KLF5 genomic alterations in tumorigenesis |
UNIVERSITY OF UTAH |
Xiaoyang Zhang |
|
| Submission date |
Mar 31, 2020 |
| Last update date |
Mar 10, 2021 |
| Contact name |
Xiaoyang Zhang |
| Organization name |
University of Utah
|
| Lab |
Zhang lab
|
| Street address |
1950 CIrcle of Hope
|
| City |
Salt Lake City |
| ZIP/Postal code |
84112 |
| Country |
USA |
| |
|
| Platforms (3) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
| GPL21697 |
NextSeq 550 (Homo sapiens) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
|
| Samples (20)
|
|
| This SubSeries is part of SuperSeries: |
| GSE147856 |
To study the function of KLF5 in squamous cancers and gastrointestinal cancers |
|
| Relations |
| BioProject |
PRJNA622288 |
| SRA |
SRP254800 |
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