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| Status |
Public on Apr 02, 2020 |
| Title |
A combinatorial code of transcription factors specifies subtypes of visual motion-sensing neurons in Drosophila |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Direction-selective T4/T5 neurons exist in four subtypes, each tuned to visual motion along one of the four cardinal directions. Along with their directional tuning, neurons of each T4/T5 subtype orient their dendrites and project their axons in a subtype-specific manner. Directional tuning, thus, appears strictly linked to morphology in T4/T5 neurons. How the four T4/T5 subtypes acquire their distinct morphologies development remains largely unknown. Here, we investigated when and how the dendrites of the four T4/T5 subtypes acquire their specific orientations, and profiled the transcriptomes of all T4/T5 neurons during this process. This revealed a simple and stable combinatorial code of transcription factors defining the four T4/T5 subtypes during their development. Changing the combination of transcription factors of specific T4/T5 subtypes resulted in predictable and complete conversions of subtype-specific properties, i.e. dendrite orientation and matching axon projection pattern. Therefore, a combinatorial code of transcription during factors coordinates the development of dendrite and axon morphologies to generate anatomical specializations differentiating subtypes of T4/T5 motion-sensing neurons.
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| |
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| Overall design |
Single-cell transcriptomic analysis of T4/T5 subtypes during different developmental stages
|
| Web link |
https://dev.biologists.org/content/early/2020/04/01/dev.186296
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| |
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| Citation(s) |
32238425 |
| Submission date |
Apr 02, 2020 |
| Last update date |
Apr 05, 2020 |
| Contact name |
Nikolai Li-Heng Hoermann |
| E-mail(s) |
hoermann@neuro.mpg.de
|
| Organization name |
Max Planck Institute of Neurobiology
|
| Department |
Circuits-Computation-Models
|
| Street address |
Am Klopferspitz 18
|
| City |
Martinsried |
| State/province |
Bavaria |
| ZIP/Postal code |
82152 |
| Country |
Germany |
| |
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| Platforms (1) |
| GPL19132 |
Illumina NextSeq 500 (Drosophila melanogaster) |
|
| Samples (10)
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| Relations |
| BioProject |
PRJNA622762 |
| SRA |
SRP255018 |
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