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| Status |
Public on Jul 31, 2020 |
| Title |
RNA sequencing of visceral fat tissue and E0771 tumor tissue harvested from WT and fat specific PD-L1 KO host |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
To study the transcriptome differences of tumor transplanted into WT and KO host, E0771 tumors harvested from WT and KO host were extracted for total RNA and mRNA sequencing was performed. To study the transcriptome differences of WT and KO fat tissue, visceral fat tissue were harvested from obese WT and KO mice and total RNA was extracted and sequenced.
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| |
|
| Overall design |
Tumor and visceral fat tissue were harvested and snap frozen for total RNA extraction. mRNA library construction and seuqencing were performed.
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| |
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| Contributor(s) |
Wu B, Chiang H, Li R |
| Citation(s) |
32817394 |
| Submission date |
Apr 06, 2020 |
| Last update date |
Aug 29, 2020 |
| Contact name |
Rong Li |
| Organization name |
The George Washington University
|
| Department |
Biochemistry and Molecular Medicine
|
| Street address |
2300 I Street
|
| City |
Washington |
| State/province |
DC |
| ZIP/Postal code |
20037 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL21493 |
Illumina HiSeq 3000 (Mus musculus) |
|
| Samples (13)
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| Relations |
| BioProject |
PRJNA623345 |
| SRA |
SRP255390 |
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