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Series GSE148160 Query DataSets for GSE148160
Status Public on Oct 31, 2021
Title Expression data from Luteolin-treated human Neural Stem Cells (hNSCs)
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Gene expression profiling reveals a potential role of Luteolin in human neuronal stem cells (hNSCs) differentiation .
hNSCs purchased from Gibco were treated with 1 μM verbenalin for 24 hours. Microarray gene expression profiling was conducted for biological replicates of hNSCs cultured in differentiation cell culture medium supplemented with Luteolin for 24 hours and untreated control cells cultured in differentiation cell cultured medium .
 
Overall design RNA was extracted using Isogen (Nippon Gene Co. Ltd., Toyama, Japan). The integrity of RNA was quantified using NanoDrop 2000 spectrophotometer (Thermo Scientific, Wilmington, DE, USA). RNA samples were prepared for gene expression profiling analysis with GeneChip® 3' Expression Arrays using 3' IVT PLUS Reagent Kit (Affymetrix Inc., Santa Clara, CA, USA). One hundred ng of total RNA from each sample was used to generate amplified and biotinylated complementary RNA (cRNA) from poly (A) RNA in a total RNA sample according to the user manual. IVT Incubation time was 16 hour. GeneAtlas® Hybridization, Wash and Stain Kit was used for hybridizing 3' IVT Array Strips according to the user manual (P/N 08-0306). Human genome array strips (HG-U219) were hybridized for 16 hours in a 45oC incubator, washed and stained and finally imaging was done with the GeneAtlas Fluidics and Imaging Station.
 
Citation(s) 34790666
Submission date Apr 06, 2020
Last update date Nov 24, 2021
Contact name Mariem Achour
E-mail(s) achourmariem@gmail.com
Organization name Alliance for Research on the Mediterranean and North Africa
Street address 1-1-1 Tennodai
City Tsukuba
State/province Ibaraki
ZIP/Postal code 305-8572
Country Japan
 
Platforms (1)
GPL13667 [HG-U219] Affymetrix Human Genome U219 Array
Samples (6)
GSM4455278 hNSCs+ Differentiation medium rep1
GSM4455279 hNSCs+ Differentiation medium rep2
GSM4455280 hNSCs+ Differentiation medium rep3
Relations
BioProject PRJNA623355

Download family Format
SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE148160_RAW.tar 7.6 Mb (http)(custom) TAR (of CEL, CHP)
Processed data included within Sample table
Processed data provided as supplementary file

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