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| Status |
Public on Aug 05, 2021 |
| Title |
Gene expression in CD21low and CD21pos B cells from CVID patients |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
A subgroup of CVID patients presents with a loss of switched memory B cells and an accumulation of CD21low B cells.RNAseq data from CVID derived CD21low and CD21pos B cells were compared to naive CD21pos B cells in Healthy controls. The data reveal a distinct gene expression profile in CD21low B cells compared to HD.
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| Overall design |
CD21 low and CD21pos B cells were sorted from freshly isolated PBMCs of CVID patients and healthy controls. Sorting included gating on CD19pos, CD27neg, IgDpos naive B cells and CD21low and CD21pos B cells were differentiated by low expression of CD21 and CD38 or high expression of CD21 and intermediate to low levels of CD38 respectively and cells were processed for RNA isolation.
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| Contributor(s) |
Keller B, Warnatz K, Landry J |
| Citation(s) |
34623902 |
| Submission date |
Apr 06, 2020 |
| Last update date |
Nov 04, 2021 |
| Contact name |
Baerbel Keller |
| Organization name |
University Medical Center Freiburg
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| Department |
Department of Rheumatology and clinical immunology
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| Lab |
Warnatz Lab
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| Street address |
Breisacherstr.115
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| City |
Freiburg |
| ZIP/Postal code |
79106 |
| Country |
Germany |
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| Platforms (1) |
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| Samples (7)
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| Relations |
| BioProject |
PRJNA623360 |
| SRA |
SRP255419 |
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