|
| Status |
Public on Dec 22, 2010 |
| Title |
A serine protease inhibitor induces necrosis of infected macrophages within granulomas during activation of tuberculosis |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
Granulomas function in humans during tuberculosis by focusing production of host antimicrobial factors against the causative bacterial agent Mycobacterium tuberculosis to contain infection. We show that mice unable to produce nitric oxide –itself an important antimicrobial molecule- demonstrate functional granulomas in the lung able to control infection after dermal infection. Disease in the lung was activated by administration of neutralising antibody against either TNF-α, which disrupted granuloma integrity, or INF-γ, which resulted in development of caseous necrosis within granulomas reminiscent of active human tuberculosis. In the latter case, the serpin protease inhibitor serpinb3a and its target protease, cathepsin G are highly expressed in cells local to necrotic regions in granulomas and serpinb3a induces necrosis of infected macrophages independently of cathepsin G binding. Therefore a single host protein is capable of inducing necrosis and bacterial growth during intracellular infection.
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| Overall design |
Microarray experiments were performed as dual-color hybridizations on Agilent mouse whole genome catalog 44K arrays. To compensate for dye-specific effects, a dye-reversal color-swap was applied.
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| Contributor(s) |
Reece ST, Kaufmann SH, Mollenkopf H |
| Citation(s) |
20679732 |
| Submission date |
Feb 13, 2009 |
| Last update date |
Dec 06, 2012 |
| Contact name |
Hans-Joachim Mollenkopf |
| E-mail(s) |
mollenkopf@mpiib-berlin.mpg.de
|
| Phone |
+49 30 28460 482
|
| Organization name |
Max-Planck-Institute for Infection Biology
|
| Lab |
Microarray/Genomics Core Facility
|
| Street address |
Charitéplatz 1
|
| City |
Berlin |
| ZIP/Postal code |
10117 |
| Country |
Germany |
| |
|
| Platforms (1) |
| GPL2872 |
Agilent-012694 Whole Mouse Genome G4122A (Feature Number version) |
|
| Samples (8)
|
| GSM371027 |
B6 d30 lung vs. B6 d30 aIFNg lung |
| GSM371108 |
iNOS d30 lung vs. iNOS d30 aIFNg lung |
| GSM371109 |
iNOS d30 aIFNg lung vs. iNOS d30 lung |
| GSM371110 |
iNOS d60 lung vs. iNOS d60 aIFNg lung |
| GSM371111 |
iNOS d60 aIFNg lung vs. iNOS d60 lung |
|
| Relations |
| BioProject |
PRJNA112061 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE14826_RAW.tar |
70.0 Mb |
(http)(custom) |
TAR (of XML) |
| GSE14826_US22502595_251269418667_S01_H.tif |
201.1 Mb |
(ftp)(http) |
TIFF |
| GSE14826_US22502595_251269418667_S01_L.tif |
201.1 Mb |
(ftp)(http) |
TIFF |
| GSE14826_US22502595_251269418668_S01_H.tif |
201.1 Mb |
(ftp)(http) |
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| GSE14826_US22502595_251269418668_S01_L.tif |
201.1 Mb |
(ftp)(http) |
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| GSE14826_US22502595_251269418947_S01_H.tif |
201.1 Mb |
(ftp)(http) |
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| GSE14826_US22502595_251269418947_S01_L.tif |
201.1 Mb |
(ftp)(http) |
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| GSE14826_US22502595_251269418948_S01_H.tif |
201.1 Mb |
(ftp)(http) |
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| GSE14826_US22502595_251269418948_S01_L.tif |
201.1 Mb |
(ftp)(http) |
TIFF |
| GSE14826_US22502595_251269418949_S01_H.tif |
201.1 Mb |
(ftp)(http) |
TIFF |
| GSE14826_US22502595_251269418949_S01_L.tif |
201.1 Mb |
(ftp)(http) |
TIFF |
| GSE14826_US22502595_251269419652_S02_H.tif |
201.1 Mb |
(ftp)(http) |
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| GSE14826_US22502595_251269419652_S02_L.tif |
201.1 Mb |
(ftp)(http) |
TIFF |
| GSE14826_US22502595_251269419653_S02_H.tif |
201.1 Mb |
(ftp)(http) |
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| GSE14826_US22502595_251269419653_S02_L.tif |
201.1 Mb |
(ftp)(http) |
TIFF |
| GSE14826_US22502595_251269419654_S01_H.tif |
201.1 Mb |
(ftp)(http) |
TIFF |
| GSE14826_US22502595_251269419654_S01_L.tif |
201.1 Mb |
(ftp)(http) |
TIFF |
| Processed data included within Sample table |
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