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| Status |
Public on Apr 24, 2020 |
| Title |
Microglia transcriptome in a rat model of ischemic stroke |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by array
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| Summary |
Microglia are key regulators of inflammatory response after stroke and brain injury. Here we profiled the microglia transcriptome isolated from a spontaneously hypertensive rat model of focal cerebral ischemia.
We identified an extensive and persistent upregulation of anti-inflammatory M2-like patterns after stroke and a mild up-regulation of pro-inflammatory M1-like patterns at later stage. We also found that younger brains showed larger microglial response than middle aged brains. Moreover, beyond the standard M1/M2 dichotomy, a wide spectrum of novel microglial polarization states was activated in response to stroke, particularly the phenotypes related to Tlr2 and dietary fatty acids stimulation.
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| Overall design |
We analyzed microglial gene expression change from 3 to 14 days after stroke onset and compared the activation pattern between 3-month and 12-month old brains.
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| Web link |
https://0-doi-org.brum.beds.ac.uk/10.1177/0271678X20932870
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| Contributor(s) |
Deng W, Mandeville E, Terasaki Y, Li W, Ning M, Lo EH, Xing C |
| Citation(s) |
33208001 |
| Submission date |
Apr 08, 2020 |
| Last update date |
Oct 01, 2021 |
| Contact name |
Wenjun Deng |
| E-mail(s) |
WDENG@mgh.harvard.edu
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| Organization name |
Massachusetts General Hospital
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| Street address |
175 Cambridge street
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| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02114 |
| Country |
USA |
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| Platforms (1) |
| GPL6247 |
[RaGene-1_0-st] Affymetrix Rat Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (30)
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| Relations |
| BioProject |
PRJNA623992 |
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