 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Feb 13, 2010 |
| Title |
Madin Darby Canine Kidney (MDCK) Cells: Early & Late Passage |
| Organism |
Canis lupus familiaris |
| Experiment type |
Expression profiling by array
|
| Summary |
The paracellular passage of ions in epithelial systems is dictated by the repertoire of tight junction (TJ) proteins, the number and architecture of apicolateral TJ strands, and the interaction of TJ proteins with the cytoskeleton. To investigate the relative contribution of these factors and to explore their regulation, we examined a passage number dependent phenotypic change in Madin Darby Canine Kidney (MDCK) cells iteratively passaged at low density in which dilution potentials of the monolayer abruptly decreased but transepithelial resistance (TER) progressively increased. Here, we report that the phenotypic transition involved a decrease surface expression of peanut agglutinin (PNA), a decrease in steady state levels of claudin-1 and a slight decrease in E-cadherin expression in the context of an apparently claudin-2-negative system. Late passage cells grew in more densely packed monolayers and exhibited characteristics of a competent, functional cultured monolayer including inducible vectorial ion transport in response to norepinephrine (NE) and sphere formation in three dimensional laminin gel culture. Microarray analysis of gene expression levels in early and late passage cells revealed an increase in transcript abundance for SGK and GLUT-3, and a reduction Id-3. The steady state protein expression levels of GLUT-3, however, were found to be equivalent. These findings suggest that the expression of claudin-2 in MDCK cells could be contingent on signaling via lateral cell contacts and that claudin-1, in the context of sustained claudin-8 expression, could function as a negative regulator of overall TER.
|
| |
|
| Overall design |
3 biological replicates of early and late passage were run on Affymetrix chips. the replicates were averaged then compared.
|
| |
|
| Contributor(s) |
Young EF, Quinn D, Aris V, Ghanny S, Soteropoulos P, Davies RJ |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Feb 13, 2009 |
| Last update date |
Mar 20, 2012 |
| Contact name |
Saleena Ghanny |
| E-mail(s) |
ghannysa@njms.rutgers.edu
|
| Organization name |
Rutgers University
|
| Street address |
185 South Orange Ave
|
| City |
Newark |
| ZIP/Postal code |
07103 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL3738 |
[Canine_2] Affymetrix Canine Genome 2.0 Array |
|
| Samples (6)
|
| GSM371279 |
MDCK-Early Passage-biological replicate1 |
| GSM371281 |
MDCK-Early Passage-biological replicate2 |
| GSM371283 |
MDCK-Early Passage-biological replicate3 |
| GSM371286 |
MDCK-Late Passage-biological replicate1 |
| GSM371287 |
MDCK-Late Passage-biological replicate2 |
| GSM371288 |
MDCK-Late Passage-biological replicate3 |
|
| Relations |
| BioProject |
PRJNA112103 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE14837_RAW.tar |
24.3 Mb |
(http)(custom) |
TAR (of CEL, CHP) |
| Processed data included within Sample table |
| Processed data provided as supplementary file |
|
|
|
|
 |
Less...
More...