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| Status |
Public on Sep 30, 2009 |
| Title |
Gene expression changes by 2,4-dinitrochlorobenzene in human bulge-derived keratinocytes |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
In the epidermis, keratinocytes are involved in physical and first-line immune protection of the host. In this work, we analyzed molecular responses after the addition of contact sensitizers, 2,4-dinitrochlorobenzene (DNCB) using cultured human keratinocytes from the bulge region of a plucked hair follicle (bulge-derived keratinocytes; BDKs) in comparison with neonatal human epidermal keratinocytes NHEK and human monocytic leukemia THP-1. The changes in gene expression by the treatment of DNCB in BDKs were different from those in THP-1. Many genes orchestrating keratinocyte differentiation, which interact TGF-β and BMP signaling pathway, were significantly up-regulated in response to DNCB.
Keywords: Comparison analysis of gene expression changes by the treatment of DNCB
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| Overall design |
three cell strains composed of four samples; two BDKs established from different donors, NHEK, and THP-1. One replicate per array.
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| |
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| Contributor(s) |
Yoshikawa Y, Sasahara Y, Kitano Y, Kanazawa N, Shima H, Hashimoto-Tamaoki T |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Feb 13, 2009 |
| Last update date |
Jan 23, 2019 |
| Contact name |
Yoshie Yoshikawa |
| E-mail(s) |
yoshiey@hyo-med.ac.jp
|
| Phone |
+81-798-45-6587
|
| Organization name |
Hyogo College of Medicine
|
| Department |
Genetics
|
| Street address |
1-1 Mukogawa-cho
|
| City |
Nishinomiya |
| State/province |
Hyogo |
| ZIP/Postal code |
663-8501 |
| Country |
Japan |
| |
|
| Platforms (1) |
| GPL6480 |
Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Probe Name version) |
|
| Samples (4)
|
| GSM371289 |
BDK established from donor II_DNCB treated_4.5 hr |
| GSM371290 |
BDK established from donor I_DNCB treated_4.5 hr |
| GSM371291 |
NHEK_DNCB treated_4.5 hr |
|
| Relations |
| BioProject |
PRJNA112105 |
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