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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Aug 18, 2009 |
| Title |
The effect of miRNA overexpression on gene expression profile of MCF-7 cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
To identify microRNAs impacting estrogen receptor ERα expression in breast cancer, we have screened ER-positive breast cancer cells with a library of pre-miRs, and systematically monitored the ERα expression by protein lysate microarrays. There was a significant enrichment of the in silico predicted ERα targeting microRNAs among the hits. The most potent pre-miRs miR-18a/b, miR-193b, miR-206, and miR-302c, were confirmed to directly target ERα and to repress estrogen-responsive genes. The effect of miRNA overexpression on gene expression profile of MCF-7 cells was studied. Furthermore, miR-18a and miR-18b showed increased expression in ERα-negative as compared to ERα-positive clinical tumors. In summary, we present systematic and direct functional and correlative clinical evidence on microRNAs inhibiting ERα signaling in breast cancer.
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| Overall design |
MCF-7 cells (300 000 per well on 6-well plates) were transfected with an siRNA for ERα or with Ambion pre-miR™ constructs for miR-18a, miR-193b, miR-206, miR-302c, or pre-miR negative control #1 (scrambled pre-miR) at 20 nM, and incubated for 24h.
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| Contributor(s) |
Leivonen S, Mäkelä R, Östling P, Kohonen P, Haapa-Paananen S, Kleivi K, Enerly E, Aakula A, Hellström K, Sahlberg N, Kristensen VN, Børresen-Dale A, Saviranta P, Perälä M, Kallioniemi O |
| Citation(s) |
19684618 |
| Submission date |
Feb 16, 2009 |
| Last update date |
Feb 15, 2013 |
| Contact name |
Pekka Juhani Kohonen |
| Organization name |
Karolinska Institutet
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| Department |
IMM Institute of Environmental Medicine
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| Street address |
Nobelsväg 13, Box 210
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| City |
Stockholm |
| ZIP/Postal code |
SE-171 77 |
| Country |
Sweden |
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| Platforms (1) |
| GPL6102 |
Illumina human-6 v2.0 expression beadchip |
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| Samples (11)
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| Relations |
| BioProject |
PRJNA112123 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE14847.txt.gz |
12.5 Mb |
(ftp)(http) |
TXT |
| GSE14847_RAW.tar |
5.8 Mb |
(http)(custom) |
TAR |
| Processed data included within Sample table |
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