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Status |
Public on Apr 13, 2020 |
Title |
bT87Q-globin gene therapy reduces sickle hemoglobin production, allowing for ex vivo anti-sickling activity in human erythroid cells |
Organism |
Homo sapiens |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
We performed RNA-seq analysis on immortalized human erythroid cell line (HUDEP-2), that had been engineered to carry the sickle cell disease (SCD) mutation (sHUDEP-2), and on healthy donor mobilized CD34+ derived cells after transduction with a control GFP lentivirus and lentivirus expressing βT87Q-globin. Our results show activation of inflammation- and proliferation-related programs, suggesting minimal changes of background gene expression except for βT87Q-globin expression and endogenous β/βs-globin suppression.
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Overall design |
RNA-seq was performed on non-transduced CD34+ derived human primary cells and on CD34+ derived human primary cells transduced with a control GFP lenitviral vector or βT87Q-globin lentiviral vector at the CD71high/CD235ahigh stage of erythroid development (day 9 of culture) and on non-transduced sHUDEP-2 cells and on sHUDEP-2 cells transduced with a control GFP or βT87Q-globin lentiviral vector at the CD71high/CD235ahigh stage (day 6 of culture).
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Contributor(s) |
Gudmundsdottir B, Tisdale JF, Pirooznia M |
Citation(s) |
32405513 |
Submission date |
Apr 12, 2020 |
Last update date |
May 18, 2020 |
Contact name |
Mehdi Pirooznia |
E-mail(s) |
pirooznia@gmail.com
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Phone |
410-340-6052
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Organization name |
National Institutes of Health
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Street address |
12 SOUTH DR
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City |
Bethesda |
State/province |
MD |
ZIP/Postal code |
20892 |
Country |
USA |
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Platforms (1) |
GPL21290 |
Illumina HiSeq 3000 (Homo sapiens) |
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Samples (6)
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GSM4473188 |
Non-transduced sHUDEP-2 cells |
GSM4473189 |
sHUDEP-2 cells transduced with GFP-lentivirus (MOI50) |
GSM4473190 |
sHUDEP-2 cells transduced with βT87Q-lentivirus (MOI50) |
GSM4473191 |
Non-transduced human CD34+ cells |
GSM4473192 |
human CD34+ cells transduced with GFP-lentivirus (MOI50) |
GSM4473193 |
human CD34+ cells transduced with βT87Q-lentivirus (MOI50) |
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Relations |
BioProject |
PRJNA624891 |
SRA |
SRP256249 |