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| Status |
Public on Jul 31, 2020 |
| Title |
Single cell RNA-seq reveals high heterogeneity among human γδ T cells. |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
Other
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| Summary |
Human γδ T cells take a small but important part in the immune system. Human γδ T cells are usually categorized by the V gene of their T cell receptor (TCR) δ chain; most of which are Vδ1+ or Vδ2+. Naive γδ T cells are often found from neonates and cytotoxic γδ T cells (γδCTLs) are frequent in adults. However, phenotypes and the developmental programs of human γδ T cells are not fully clear. Here, by single-cell RNA sequencing (sc-RNAseq) of transcriptome and T cell receptor (sc-TCRseq) on γδ T cells from neonates and adults, we revealed human γδ T cells can be divided as naïve T cells, γδCTLs, and γδNKT cells which are featured by pro-Vδ1, Vδ1, and Vδ2 TCR repertoire, respectively. γδNKT cells can be further described as γδNKT-1, γδNKT-2, and γδNKT-17 cells.
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| Overall design |
Sort ϒδT cells from neonatal cord blood and adult peripheral blood, then do 5'-RNA single cell transcriptome and TCR sequening (10x genomics).
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| Contributor(s) |
Tan L, Fichtner A, Ravens S, Prinz I |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Apr 26, 2020 |
| Last update date |
Oct 06, 2020 |
| Contact name |
Immo Prinz |
| E-mail(s) |
i.prinz@uke.de
|
| Organization name |
University medical center Hamburg Eppendorf
|
| Department |
Institute of Systems Immunology
|
| Street address |
Falkenried 94
|
| City |
Hamburg |
| ZIP/Postal code |
20251 |
| Country |
Germany |
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| Platforms (2) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA628640 |
| SRA |
SRP258695 |
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