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| Status |
Public on May 09, 2020 |
| Title |
Voltage-gated Sodium Channel SCN5A Promotes Tumor Progression and Enhances Chemosensitivity to 5-Fluorouracil in Colorectal Cancer |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
SCN5A was reported to promote proliferation and invasiveness potential in colorectal cancer, yet the mechanism was not clarified, and little was known about its association with chemosensitivity to 5-fluorouracil. In the current study, elevated SCN5A expression were associated with poor prognosis and metastasis in colorectal cancers, whereas stage II-III patients with up-regulated SCN5A expression were more likely to benefit from 5-fluorouracil-based adjuvant chemotherapy. SCN5A could promote proliferation and invasiveness potential through regulating cell cycle and epithelial-mesenchymal transition in colorectal cancer cells. Furthermore, SCN5A regulated Ras signaling by stabilizing KRas-calmodulin complex through Ca2+ influx. Apart from that, under 5-fluorouracil treatment, SCN5A promoted calmodulin-dependent apoptosis in colorectal cancers.
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| Overall design |
This study was carried out in Sun Yat-Sen University Cancer Center. CRC cases receiving surgical treatment were investigated, and IHC scores of SCN5A were determined.We also obtained Online data on SCN5A expression and clinical data from The Cancer Genome Atlas (TCGA) as well as the GEO data sets. SCN5A knockdown SW480 and DLD1 cells were constructed to investigate the regulatory role of SCN5A in cellular phenotypes. RNA sequencing was applied to explore the underlying mechanism. To demonstrate the association between SCN5A and chemosensitivity to 5-FU, cell apoptosis assay was conducted following 5-fluorouracil treatment.
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| Contributor(s) |
Sui Q, Peng J, Han K, Lin J, Zhang R, Ou Q, Deng Y, Zhou W, Kong L, Tang J, Xiao B, Li Y, Yu L, Fang Y, Ding P, Pan Z |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
May 08, 2020 |
| Last update date |
May 09, 2020 |
| Contact name |
Qiaoqi Sui |
| E-mail(s) |
suiqq@sysucc.org.cn
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| Phone |
+8613602473301
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| Organization name |
Sun Yat-sen University Cancer Center
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| Street address |
Dongfeng East Road 651
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| City |
Guangzhou |
| ZIP/Postal code |
510060 |
| Country |
China |
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| Platforms (1) |
| GPL21290 |
Illumina HiSeq 3000 (Homo sapiens) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA631241 |
| SRA |
SRP260805 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE150134_processed_data_file.txt.gz |
2.1 Mb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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