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Series GSE150264 Query DataSets for GSE150264
Status Public on Jun 20, 2020
Title Reduced Brd1 expression leads to reversible depression-like behaviors and gene-expression changes in female mice [anterior cingulate cortex (aCC)]
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Background: The schizophrenia associated gene, BRD1, encodes an epigenetic regulator which chromatin interactome is enriched with genes implicated in mental health. Alterations in histone modifications and epigenetic regulation contribute to brain transcriptomic changes in affective disorders and preclinical data supports a role for BRD1 in psychopathology. However, the implications of BRD1 on affective pathology remains poorly understood.
Methods: We assessed affective behaviors and associated neurobiology in Brd1+/- mice along with their responses to Fluoxetine and Imipramine. This involves behavioral, neurostructural, and neurochemical characterizations along with regional cerebral gene expression profiling combined with integrative functional genomic analyses.
Results: Here we report behavioral changes in female Brd1+/- mice with translational value to depressive symptomatology that can be alleviated by administration of antidepressant medications. Behavioral changes are accompanied by altered brain morphometry and imbalances in monoaminergic systems. In accordance, gene expression changes across brain tissues reveal altered neurotransmitter signaling and cluster in functional pathways associated with depression including ‘Adrenergic-, GPCR-, cAMP-, and CREB/CREM-signaling’. Integrative gene expression analysis specifically links changes in amygdaloid intracellular signaling activity to the behavioral treatment response in Brd1+/- mice.
Conclusions: Collectively, our study highlights the importance of BRD1 as a modulator of affective pathology and adds to our understanding of the molecular mechanisms underlying affective disorders and their treatment response.
 
Overall design mRNA profiles of right striatum, caudate putamen and anterior cingulate cortex of 8-11 weeks-old female wild type (WT) and Brd1+/- mice, either untreated or treated with antidepressants imipramine or fluoxetine, were generated by RNA-sequencing, using BGISEQ-500, in order to identify diffentially expressed genes between groups.
 
Contributor(s) Rajkumar A, Qvist P, Donskov J
Citation(s) 32681022
Submission date May 11, 2020
Last update date Jul 27, 2020
Contact name Per Qvist
E-mail(s) per.q@biomed.au.dk
Organization name Aarhus University
Department Biomedicine
Street address Hoegh Guldbergs Gade 10, Building 1116-460
City Aarhus c
State/province Denmark
ZIP/Postal code 8000
Country Denmark
 
Platforms (1)
GPL23479 BGISEQ-500 (Mus musculus)
Samples (36)
GSM4544477 Brd1+/- IMN 1 [anterior cingulate cortex (aCC)]
GSM4544478 WT vehicle 1 [anterior cingulate cortex (aCC)]
GSM4544479 WT vehicle 2 [anterior cingulate cortex (aCC)]
This SubSeries is part of SuperSeries:
GSE150265 Reduced Brd1 expression leads to reversible depression-like behaviors and gene-expression changes in female mice
Relations
BioProject PRJNA631693
SRA SRP261095

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Supplementary file Size Download File type/resource
GSE150264_TPM_all_data_aCC.csv.gz 4.1 Mb (ftp)(http) CSV
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Raw data are available in SRA
Processed data are available on Series record

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