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Series GSE15069 Query DataSets for GSE15069
Status Public on Apr 30, 2009
Title Inhibitor trials in chondrocytes - MAS 5.0 normalization
Organism Mus musculus
Experiment type Expression profiling by array
Summary Objectives: To identify similarities and differences in gene expression data in the MEK/ERK and PI3K pathways and to determine how histone modification affects these same pathways. Goal: To identify and functionally characterize novel targets of these signaling pathways in the context of chondrocyte differentiation.

Keywords: Treatment, signaling, one-colour array, signaling pathway comparison
Overall design Cartilage derived from the hind limbs of CD1 mice staged at 15.5 days of embryonic development were dissected and plated in primary chondrocyte monolayer cultures. Cells were treated with MEK/ERK pathway inhibitors (SB202190 and UO126), a PI3K inhibitor (LY294002), a histone deacetylase inhibitor (Trichostatin A) and the vehicle control (DMSO) for 24 hours. Total RNA was isolated from these samples and hybridized to Affymetrix MOE 430 2.0 chips at the London Regional Genomics facility.
A total of 15 genechips were analyzed between 4 treatments and the vehicle (DMSO) control. Three biological replicates per treatment were executed.

Contributor(s) James CG, Ulici V, Beier F, Stanton L
Citation(s) 20111593
Submission date Mar 02, 2009
Last update date Feb 11, 2019
Contact name veronica ulici
Phone 1-519-661-3387
Organization name University of Western Ontario
Department Physiology and Pharmacology
Lab Dr. Frank Beier
Street address 1151 Richmond Street
City London
State/province Ontario
ZIP/Postal code N6A5C1
Country Canada
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (15)
GSM376950 DMSO1_24HR
GSM376951 DMSO2_24HR
GSM376952 DMSO3_24HR
BioProject PRJNA114847

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE15069_RAW.tar 63.8 Mb (http)(custom) TAR (of CEL, CHP)
Raw data provided as supplementary file
Processed data included within Sample table
Processed data provided as supplementary file

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