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Series GSE150765 Query DataSets for GSE150765
Status Public on Oct 29, 2020
Title Small RNA libraries from epidermal stem cell (ESC) and fibroblast (Fb) derived exosomes
Organism Mus musculus
Experiment type Non-coding RNA profiling by high throughput sequencing
Summary We employed high-throughput sequencing to profile the non-coding RNA content of primary ESC and Fb derived exosomes. All 6 libraries were sequenced to a comparable depth (ESC1: 12.3M, ESC2: 14M, ESC3: 12.8M, FB1: 14.7M, FB2: 13.7M, FB3: 12.3M) and exhibited a very low proportion of adapter-only reads or fragments shorter than 17bp (4.4 ± 0.5% ), showing efficient fragment incorporation during library preparation. All libraries had high small RNA content (>50% successfully aligned and assigned to small RNAs). ESC libraries had significantly higher small RNA content (ESC: 84.5 ± 0.005% vs FB: 60.5 ± 0.05%, p<0.05, two-sided Welch’s t-test). ESC samples show remarkably higher proportions of miRNAs (~9-fold higher, p=3.6*10-5 ) and snRNAs (~12-fold higher, p=0.002) compared to Fb. Fibroblasts exhibited higher RPM of reads mapping on lncRNAs (4-fold, p=0.052)
 
Overall design Non-coding RNA-seq was performed on three biological replicates each of mouse epidermal stem cell (ESC) and dermal fibroblast (Fb) derived exosomes
 
Contributor(s) Theocharidis G, Vlachos IS
Citation(s) 35181300
Submission date May 18, 2020
Last update date Feb 22, 2022
Contact name Aristidis Veves
E-mail(s) aveves@bidmc.harvard.edu
Phone 6176627075
Organization name Beth Israel Deaconess Medical Center
Lab Rongxiang Xu, MD, Center for Regenerative Therapeutics
Street address 330 Brookline Ave
City Boston
State/province MA
ZIP/Postal code 02215
Country USA
 
Platforms (1)
GPL21626 NextSeq 550 (Mus musculus)
Samples (6)
GSM4558299 ESC1
GSM4558300 ESC2
GSM4558301 ESC3
Relations
BioProject PRJNA633535
SRA SRP262156

Download family Format
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE150765_RAW.tar 520.0 Kb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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