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| Status |
Public on May 01, 2021 |
| Title |
Expression profiles of skeletal muscle from transgenic mouse with liver specific overexpression |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Purpose: Next-generation sequencing (NGS) has revolutionized systems-based analysis of cellular pathways. The goals of this study are to evaluate the effects of liver-specific E4BP4 overexpression under mouse albumin promoter on the muscle glucose and lipid metabolism.
Methods: We generated transgenic mice (TG) with liver-specific E4BP4 overexpression. Gasrocnemius muscles were isolated at Zeitgeber Time (ZT) 0 or 12 from transgenic mice and WT littermates, and total RNA was extracted. Muscle RNA profiles were generated by deep sequencing for four groups with three mouse samples each.
Results: There were no significant differences between WT and transgenic mice at both ZT0 and 12.
Conclusions: Muscle metabolism was not altered at the transcription level by liver specific overexpression of E4BP4.
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| Overall design |
Muscle mRNA profiles of 8-week old wild type mice and transgenic mice with liver-specific E4BP4 overexpression
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| Contributor(s) |
Ohta Y, Taguchi A, Tanizawa Y |
| Citation missing |
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| Submission date |
May 21, 2020 |
| Last update date |
May 01, 2021 |
| Contact name |
Akihiko Taguchi |
| E-mail(s) |
a.tgc@yamaguchi-u.ac.jp
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| Organization name |
Yamaguchi University
|
| Street address |
1-1-1 minamikogushi
|
| City |
Ube |
| State/province |
Yamaguchi |
| ZIP/Postal code |
7558505 |
| Country |
Japan |
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| Platforms (1) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA634327 |
| SRA |
SRP262621 |
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