|
| Status |
Public on May 18, 2021 |
| Title |
Effect of methanol fixation on single-cell RNA sequencing data |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We report how methanol fixation influences transcriptome profile in single cell RNA-seq. We generatad Smart-seq2 data from two cell lines, and both live and fixed cells from each cell line were processed and analyzed to illustrate fixaiton effect.
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| |
|
| Overall design |
We used Smart-seq2 to generate single cell RNA-seq data from two cell lines: HCT-116 and HepG2. Both live and fixed cells were generated from each cell line.
|
| |
|
| Contributor(s) |
Wang X |
| Citation(s) |
34090348 |
| Submission date |
May 21, 2020 |
| Last update date |
Jun 15, 2021 |
| Contact name |
Xinlei Wang |
| E-mail(s) |
xwangda@connect.ust.hk
|
| Organization name |
HKUST
|
| Department |
LIFS
|
| Lab |
Angela Wu Lab
|
| Street address |
Clear Water Bay, Sai Kung,
|
| City |
Hong Kong |
| State/province |
Hong Kong |
| ZIP/Postal code |
999077 |
| Country |
China |
| |
|
| Platforms (1) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
|
| Samples (346)
|
|
| Relations |
| BioProject |
PRJNA634342 |
| SRA |
SRP262635 |
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