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| Status |
Public on Jul 03, 2004 |
| Title |
Human UniGene Set RZPD 1 - Zipf's Normalization |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Five microarrays from a larger dataset used to demonstrate a normalization technique base on Zipf's law. The original data-set generated using a global genome-wide cDNA clone set (Human UniGene clone set RZPD 1 - http://www.rzpd.de - Build 138, NCBI), which consisted of ~33,792 cDNA clone inserts spotted in duplicate onto membranes (18). These microarrays (n=31) were hybridized with 33P-labeled cDNA derived from total RNA extracted from biopsy material from the sigmoidal colon of normal (control, n=11), and patients with Crohn's disease (condition A, n=10) and ulcerative colitis (condition B, n=10). Microarrays were probed according to established protocols and exposed to imaging plates overnight (BAS-MS 2325) and scanned at a 50 m resolution on a FLA-3000G phosphoimager (Raytest, Germany). Image gridding was carried out using VisualGrid® software (http://www.gpc-biotech.com).
Keywords: other
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| Contributor(s) |
Lu T, Costello CM, Croucher PJ, Haesler R, Deuschl G, Schreiber S |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Jun 26, 2004 |
| Last update date |
Mar 15, 2012 |
| Contact name |
Timothy TeHua Lu |
| E-mail(s) |
t.lu@mucosa.de
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| Phone |
+0049-431-597-1617
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| Organization name |
Christian-Albrechts-University Kiel
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| Street address |
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| City |
Kiel |
| ZIP/Postal code |
D24103 |
| Country |
Germany |
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| Platforms (1) |
| GPL284 |
Human UniGene Set RZPD 1 |
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| Samples (5)
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| Relations |
| BioProject |
PRJNA90839 |
| Supplementary data files not provided |
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