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Status |
Public on May 23, 2020 |
Title |
Single-cell omics reveal human blood mononuclear CD14+ and/or CD1c+ cell heterogeneity |
Organism |
Homo sapiens |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
Dendritic cells (DC) are antigen presenting cells controlling T cell activation. In human, the diversity, ontogeny and functional capabilities of DC subsets are not fully understood. Here, we identified circulating CD88-CD1c+CD163+ DC (termed as DC3) as an immediate precursor of inflammatory CD88-CD14+CD1c+CD163+FcεRI+ DC. DC3 develop via a specific pathway, independent from the cDC-restricted (CDP) and monocyte-restricted (cMoP) progenitors, and are activated by GM-CSF. As classical DC, but unlike monocytes, DC3 drove the activation of naïve T cells. In vitro, DC3 displayed a distinctive ability to prime CD8+ T cells expressing a tissue-homing signature and the epithelial homing alpha-E integrin (CD103) through transforming growth factor-b (TGF-β) signaling. In vivo, DC3 infiltrated luminal breast cancer primary tumors and DC3 infiltration correlated positively with CD8+CD103+CD69+ tissue-resident memory T cells. Altogether, these findings define DC3 as a lineage of inflammatory DC endowed with a strong potential to regulate tumor immunity.
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Overall design |
Single cell RNAseq (scRNAseq) of human peripheral blood dendritic cells and monocytes. 3 healthy donors were equally pooled.
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Contributor(s) |
Bourdely P, Vaivode K, Anselmi G, Ramos RN, Missolo-Kousou Y, Hidalgo S, Tosselo J, Nunez N, Richer W, Vincent-Salomon A, Saxena A, Wood K, Lladser A, Piaggio E, Helft J, Guermonprez P |
Citation(s) |
32610077 |
Submission date |
May 22, 2020 |
Last update date |
Aug 24, 2020 |
Contact name |
Kristine Vaivode |
E-mail(s) |
kristine.vaivode@kcl.ac.uk
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Phone |
02078486907
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Organization name |
King's College London
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Department |
Centre for Inflammation Biology and Cancer Immunology
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Lab |
Dr Pierre Guermonprez lab
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Street address |
CIBCI, First Floor, New Hunt's House, Great Maze Pond
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City |
London |
ZIP/Postal code |
SE1 1UL |
Country |
United Kingdom |
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Platforms (1) |
GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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Samples (1) |
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This SubSeries is part of SuperSeries: |
GSE151095 |
Transcriptional and functional analysis of CD1c+ human dendritic cells identifies a CD163+ subset priming CD8+CD103+ T cells |
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Relations |
BioProject |
PRJNA634563 |
SRA |
SRP263023 |
Supplementary file |
Size |
Download |
File type/resource |
GSE151087_RAW.tar |
10.6 Mb |
(http)(custom) |
TAR (of MTX, TSV) |
SRA Run Selector |
Raw data are available in SRA |
Processed data provided as supplementary file |
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