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Series GSE151087 Query DataSets for GSE151087
Status Public on May 23, 2020
Title Single-cell omics reveal human blood mononuclear CD14+ and/or CD1c+ cell heterogeneity
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Dendritic cells (DC) are antigen presenting cells controlling T cell activation. In human, the diversity, ontogeny and functional capabilities of DC subsets are not fully understood. Here, we identified circulating CD88-CD1c+CD163+ DC (termed as DC3) as an immediate precursor of inflammatory CD88-CD14+CD1c+CD163+FcεRI+ DC. DC3 develop via a specific pathway, independent from the cDC-restricted (CDP) and monocyte-restricted (cMoP) progenitors, and are activated by GM-CSF. As classical DC, but unlike monocytes, DC3 drove the activation of naïve T cells. In vitro, DC3 displayed a distinctive ability to prime CD8+ T cells expressing a tissue-homing signature and the epithelial homing alpha-E integrin (CD103) through transforming growth factor-b (TGF-β) signaling. In vivo, DC3 infiltrated luminal breast cancer primary tumors and DC3 infiltration correlated positively with CD8+CD103+CD69+ tissue-resident memory T cells. Altogether, these findings define DC3 as a lineage of inflammatory DC endowed with a strong potential to regulate tumor immunity.
 
Overall design Single cell RNAseq (scRNAseq) of human peripheral blood dendritic cells and monocytes. 3 healthy donors were equally pooled.
 
Contributor(s) Bourdely P, Vaivode K, Anselmi G, Ramos RN, Missolo-Kousou Y, Hidalgo S, Tosselo J, Nunez N, Richer W, Vincent-Salomon A, Saxena A, Wood K, Lladser A, Piaggio E, Helft J, Guermonprez P
Citation(s) 32610077
Submission date May 22, 2020
Last update date Aug 24, 2020
Contact name Kristine Vaivode
E-mail(s) kristine.vaivode@kcl.ac.uk
Phone 02078486907
Organization name King's College London
Department Centre for Inflammation Biology and Cancer Immunology
Lab Dr Pierre Guermonprez lab
Street address CIBCI, First Floor, New Hunt's House, Great Maze Pond
City London
ZIP/Postal code SE1 1UL
Country United Kingdom
 
Platforms (1)
GPL24676 Illumina NovaSeq 6000 (Homo sapiens)
Samples (1)
GSM4566148 hPBMCs_day0
This SubSeries is part of SuperSeries:
GSE151095 Transcriptional and functional analysis of CD1c+ human dendritic cells identifies a CD163+ subset priming CD8+CD103+ T cells
Relations
BioProject PRJNA634563
SRA SRP263023

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Supplementary file Size Download File type/resource
GSE151087_RAW.tar 10.6 Mb (http)(custom) TAR (of MTX, TSV)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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