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GEO help: Mouse over screen elements for information. |
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| Status |
Public on May 23, 2020 |
| Title |
Single-cell omics reveal human GM-CSF-dependent mononuclear phagocyte subsets in humanized mice |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Dendritic cells (DC) are antigen presenting cells controlling T cell activation. In human, the diversity, ontogeny and functional capabilities of DC subsets are not fully understood. Here, we identified circulating CD88-CD1c+CD163+ DC (termed as DC3) as an immediate precursor of inflammatory CD88-CD14+CD1c+CD163+FcεRI+ DC. DC3 develop via a specific pathway, independent from the cDC-restricted (CDP) and monocyte-restricted (cMoP) progenitors, and are activated by GM-CSF. As classical DC, but unlike monocytes, DC3 drove the activation of naïve T cells. In vitro, DC3 displayed a distinctive ability to prime CD8+ T cells expressing a tissue-homing signature and the epithelial homing alpha-E integrin (CD103) through transforming growth factor-b (TGF-β) signaling. In vivo, DC3 infiltrated luminal breast cancer primary tumors and DC3 infiltration correlated positively with CD8+CD103+CD69+ tissue-resident memory T cells. Altogether, these findings define DC3 as a lineage of inflammatory DC endowed with a strong potential to regulate tumor immunity.
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| Overall design |
3 independent immunodeficient NSG mice were injected intravenously with B16 producing human GM-CSF. Lung metastasis bearing mice were engrafted with 3 independent human PBMCs. Human CD45+ cells were isolated from metastatic mouse lungs. Sorted cells from each mouse were pooled together and CD1c+ and CD14+ cells were mixed at 80:20 ratio.
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| Contributor(s) |
Bourdely P, Vaivode K, Anselmi G, Ramos RN, Missolo-Kousou Y, Hidalgo S, Tosselo J, Nunez N, Richer W, Vincent-Salomon A, Saxena A, Wood K, Lladser A, Piaggio E, Helft J, Guermonprez P |
| Citation(s) |
32610077 |
| Submission date |
May 22, 2020 |
| Last update date |
Aug 24, 2020 |
| Contact name |
Kristine Vaivode |
| E-mail(s) |
kristine.vaivode@kcl.ac.uk
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| Phone |
02078486907
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| Organization name |
King's College London
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| Department |
Centre for Inflammation Biology and Cancer Immunology
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| Lab |
Dr Pierre Guermonprez lab
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| Street address |
CIBCI, First Floor, New Hunt's House, Great Maze Pond
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| City |
London |
| ZIP/Postal code |
SE1 1UL |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (1) |
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| This SubSeries is part of SuperSeries: |
| GSE151095 |
Transcriptional and functional analysis of CD1c+ human dendritic cells identifies a CD163+ subset priming CD8+CD103+ T cells |
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| Relations |
| BioProject |
PRJNA634562 |
| SRA |
SRP263025 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE151089_RAW.tar |
1.1 Mb |
(http)(custom) |
TAR (of MTX, TSV) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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