|
| Status |
Public on Jan 13, 2021 |
| Title |
Transcriptome profiling of SUM159 and derivatives with different metastasis capability |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Triple negative breast cancer cell line SUM159 labeled with triple reporter (TR, TK-GFP-fLUC) injected into Nude mice via mammary fat pad and tail vein injection. Tumor progression is monitored by in vivo bioluminescent imaging.
Tumors from mammary and lung were isolated and cultured. These derivatives cells are further tested for their capability to develop mammary primary tumor and metastasis in lung and bone in mice.
The total RNA samples are extracted from the panel of SUM159 and derivatives and the transcriptome profiles are determined by microarray. The correlation of transcriptome of the cells to metastasis capability indicated candidate genes and regulatory pathways of metastasis of TNBC.
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| |
|
| Overall design |
Transcriptome correlation to metastasis capability of a panel of 7 cells from SUM159 origin.
|
| |
|
| Contributor(s) |
Esposito M, Wei Y |
| Citation(s) |
33723425 |
| Submission date |
May 26, 2020 |
| Last update date |
Feb 29, 2024 |
| Contact name |
Yong Wei |
| E-mail(s) |
yongwei@princeton.edu
|
| Organization name |
Princeton University
|
| Department |
Molecular Biology
|
| Lab |
Kang
|
| Street address |
LTL227 Wathington Road
|
| City |
Plainsboro |
| State/province |
NJ |
| ZIP/Postal code |
08536 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL17077 |
Agilent-039494 SurePrint G3 Human GE v2 8x60K Microarray 039381 (Probe Name version) |
|
| Samples (7)
|
| GSM4568454 |
SUM159 parental line |
| GSM4568455 |
SUM159 parental line expressing triple reporter |
| GSM4568456 |
SUM159 from 1st round mammary tumor from TR injection |
| GSM4568457 |
SUM159 from 2nd round mammary tumor from P1 reinjection |
| GSM4568458 |
SUM159 from 1st round lung met from P1 reinjection #1 |
| GSM4568459 |
SUM159 from 1st round lung met from P1 reinjection #2 |
| GSM4568460 |
SUM159 from 2nd round lung met from M1a reinjection |
|
| Relations |
| BioProject |
PRJNA635112 |
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