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| Status |
Public on Jul 05, 2021 |
| Title |
The Bromodomains of the mammalian SWI/SNF (mSWI/SNF) ATPases Brahma (BRM) and Brahma Related Gene 1 (BRG1) promote chromatin interaction and are critical for skeletal muscle differentiation |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We performed RNA-seq analysis for C2C12 myoblasts as a function of differentiation. Cells were treated with either DMSO or family VIII bromodomain-specific inhibitor PFI-3 at a concentration of 50uM. We find that PFI-3 treatment affects proper differentiation of myoblasts as compared to DMSO-treated C2C12 cells. We conclude that function of mammalian SWI/SNF bromodomains is important for regulation of myoblast differentiation.
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| Overall design |
RNA-seq data was generated from C2C12 cells induced for differentiation. Cells were treated with either DMSO or PFI-3. Samples were harvested at proliferative stage (GM) or 24h/48h post induction of differentiation (DM 24h and DM 48h). RNA from biological duplicates (for each treatment and timepoint) was extracted. Library preparation, sequencing and downstream analysis was performed by BGI Americas.
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| Contributor(s) |
Imbalzano AN, Sharma T |
| Citation(s) |
34289068 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| R01 GM056244 |
Transcription Factor Function in Chromatin |
UNIV OF MASSACHUSETTS MED SCHOOL |
ANTHONY N IMBALZANO |
| R35 GM136393 |
Regulation of gene expression by chromatin remodeling enzymes |
UNIV OF MASSACHUSETTS MED SCHOOL |
ANTHONY N IMBALZANO |
|
| Submission date |
May 26, 2020 |
| Last update date |
Aug 24, 2021 |
| Contact name |
Tapan Sharma |
| E-mail(s) |
Tapan.Sharma@umassmed.edu
|
| Organization name |
University of Massachusetts Medical School
|
| Department |
Gene Therapy Centre
|
| Lab |
Gao Lab
|
| Street address |
368 Plantation Street
|
| City |
Worcester |
| State/province |
MA |
| ZIP/Postal code |
01605 |
| Country |
USA |
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| Platforms (1) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA635143 |
| SRA |
SRP264826 |
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