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| Status |
Public on Jul 07, 2020 |
| Title |
Single-cell analysis of mouse plasmacytoid dendritic cells (pDCs) unravels their activation trajectory and their molecular regulation in vivo during a viral infection [FB5P-seq_C57Bl6] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The aim of the project is to understand the heterogeneity of murine plasmacytoid dendritic cells (pDC) for interferon I production. For this end we want to perform RNAseq on splenic pDC at ground state and also after infection of mice with MCMV at different time points.
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| Overall design |
This dataset contains data from pDC sorted from wt C57Bl/6 mice, infected or not with Murine CytoMegalo virus (MCMV). Single-cells were FACS-sorted into 7 96-well plates (plates 1 and 2 : non-infected, plates 3 to 7 : infected) and analyzed by 5'-end single-cell RNAseq following the FB5P-seq protocol.
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| Contributor(s) |
Abbas A, Vu Manh T, Dalod M, Tomasello E |
| Citation(s) |
32690951 |
| Submission date |
May 27, 2020 |
| Last update date |
Nov 09, 2020 |
| Contact name |
Thien-Phong Vu Manh |
| Organization name |
CIML
|
| Street address |
163 avenue de Luminy
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| City |
marseille |
| ZIP/Postal code |
13288 |
| Country |
France |
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| Platforms (1) |
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| Samples (7)
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| This SubSeries is part of SuperSeries: |
| GSE151248 |
Single-cell analysis of mouse plasmacytoid dendritic cells (pDCs) unravels their activation trajectory and their molecular regulation in vivo during a viral infection |
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| Relations |
| BioProject |
PRJNA635287 |
| SRA |
SRP264903 |
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