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Series GSE151367 Query DataSets for GSE151367
Status Public on Dec 29, 2020
Title MicroRNA and mRNA integrated transcriptional analysis in a Cx30-/- mouse model of non-syndromic hearing loss and deafness [mRNA]
Organism Mus musculus
Experiment type Expression profiling by array
Summary The GJB6 gene is located just 35 kb telomeric to GJB2 in the so-called nonsyndromic hearing loss and deafness locus 1 (DFNB1). Knock out mouse models confirmed that inner ear expression of their protein products, connexin 30 (Cx30) and connexin 26 (Cx26), is crucial for hearing acquisition and normal development of the organ of Corti, however the coordinated regulation mechanism of Cx26 and Cx30 expression in the cochlea remains unclear. To investigate the mechanism underlying the etiopathogenesis of DFNB1, we used a microRNA (miRNA) and mRNA integrated expression profiling analysis on Cx30 -/- mice, which represent a model for humans in which large deletions in the DFNB1 locus lead to the down-regulation of both connexins and profound deafness.
 
Overall design Messenger RNA expression profiling of cochlear tissue from Cx30 wild-type mice at P5, and their age-matched homozygous knock-out siblings (Cx30-/-) were analysed by using an 8x60K SurePrint G3 Mouse GE v2 Microarray. Four biological replicates for each of the two genotypes were used.
 
Contributor(s) Gentile G, Spampinato AG, Guarnaccia M, Cavallaro S
Citation(s) 33569381
Submission date May 28, 2020
Last update date Feb 12, 2021
Contact name Sebastiano Cavallaro
E-mail(s) sebastiano.cavallaro@cnr.it
Organization name IRIB-CNR
Street address Via Paolo Gaifami, 18
City Catania
ZIP/Postal code 95126
Country Italy
 
Platforms (1)
GPL21163 Agilent-074809 SurePrint G3 Mouse GE v2 8x60K Microarray [Probe Name version]
Samples (8)
GSM4576269 cochlear_tissue_Cx30-/-_mice_rep1 [mRNA]
GSM4576270 cochlear_tissue_Cx30-/-_mice_rep3 [mRNA]
GSM4576271 cochlear_tissue_Cx30-/-_mice_rep2 [mRNA]
This SubSeries is part of SuperSeries:
GSE151369 MicroRNA and mRNA integrated transcriptional analysis in a Cx30-/- mouse model of non-syndromic hearing loss and deafness
Relations
BioProject PRJNA635630

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE151367_RAW.tar 36.5 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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