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| Status |
Public on Mar 12, 2009 |
| Title |
Expression data from Arabidopsis seed compartments at the heart stage. |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Expression profiling by array
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| Summary |
We collected heart stage seed compartments from 7 micron paraffin sections using the Leica LMD6000 system in order to identify the mRNAs present in different compartments from seeds containing heart stage embryos. For the purposes of this study we captured 6 compartments: embryo proper, micropylar endosperm, peripheral endosperm, chalazal endosperm, chalazal seed coat and seed coat, as well sets of serial sections encompassing the entire heart stage seed.
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| Overall design |
Heart stage seed compartments were isolated using the LMD6000 system. Total RNA was amplified and hybridized with Affymetrix ATH1 Arabidopsis array for 16 samples (embryo proper, micropylar endosperm, peripheral endosperm, seed coat and whole seeds, 2 biological replicates each; chalazal endosperm, chalazal seed coat, 3 biological replicates each).
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| Contributor(s) |
Stone SL, Pelletier J, Kirkbride RC, Belmonte MF, Fei J, Hashimoto MM, Cheng C, Golberg RB, Harada JJ |
| Citation(s) |
23319655, 20385809 |
| Submission date |
Mar 09, 2009 |
| Last update date |
Aug 28, 2018 |
| Contact name |
John J Harada |
| E-mail(s) |
jjharada@ucdavis.edu
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| Phone |
530-752-0673
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| Organization name |
University of California, Davis
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| Department |
Plant Biology
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| Lab |
Harada
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| Street address |
One Shields Avenue
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| City |
Davis |
| State/province |
CA |
| ZIP/Postal code |
95616 |
| Country |
USA |
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| Platforms (1) |
| GPL198 |
[ATH1-121501] Affymetrix Arabidopsis ATH1 Genome Array |
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| Samples (16)
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| GSM378645 |
Laser capture microdissected (LCM) embryo proper at the heart stage, biological replicate 1 |
| GSM378646 |
Laser capture microdissected (LCM) embryo proper at the heart stage, biological replicate 2 |
| GSM378647 |
Laser capture microdissected (LCM) micropylar endosperm at the heart stage, biological replicate 1 |
| GSM378648 |
Laser capture microdissected (LCM) micropylar endosperm at the heart stage, biological replicate 2 |
| GSM378649 |
Laser capture microdissected (LCM) peripheral endosperm at the heart stage, biological replicate 1 |
| GSM378650 |
Laser capture microdissected (LCM) peripheral endosperm at the heart stage, biological replicate 2 |
| GSM378651 |
Laser capture microdissected (LCM) chalazal endosperm at the heart stage, biological replicate 1 |
| GSM378652 |
Laser capture microdissected (LCM) chalazal endosperm at the heart stage, biological replicate 2 |
| GSM378653 |
Laser capture microdissected (LCM) chalazal endosperm at the heart stage, biological replicate 3 |
| GSM378654 |
Laser capture microdissected (LCM) chalazal seed coat at the heart stage, biological replicate 1 |
| GSM378655 |
Laser capture microdissected (LCM) chalazal seed coat at the heart stage, biological replicate 2 |
| GSM378656 |
Laser capture microdissected (LCM) chalazal seed coat at the heart stage, biological replicate 3 |
| GSM378657 |
Laser capture microdissected (LCM) seed coat at the heart stage, biological replicate 1 |
| GSM378658 |
Laser capture microdissected (LCM) seed coat at the heart stage, biological replicate 2 |
| GSM378659 |
Laser capture microdissected (LCM) whole seeds at the heart stage, biological replicate 1 |
| GSM378660 |
Laser capture microdissected (LCM) whole seeds at the heart stage, biological replicate 2 |
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| This SubSeries is part of SuperSeries: |
| GSE12404 |
Expression data from Arabidopsis Seed Compartments at 5 discrete stages of development |
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| Relations |
| BioProject |
PRJNA114933 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE15160_RAW.tar |
30.5 Mb |
(http)(custom) |
TAR (of CEL, CHP) |
| Processed data included within Sample table |
| Processed data provided as supplementary file |
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