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| Status |
Public on Aug 07, 2020 |
| Title |
Transcriptomics of Human Liver Ischemia and Reperfusion Injury |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
For orthotopic liver transplants, ischemia-reperfusion injury (IRI) is known to compromise allograft survival. To better understand the transcriptomics of immune responses underlying liver transplant IRI, RNA-seq was performed on liver biopsies from 23 IRI+ and 17 IRI- patients, both pre-transplant and post-transplant. Genes that are differentially expressed in IRI+ samples, along with the pathways enriched for by those genes, were identified.
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| Overall design |
Needle-core liver biopsies from liver transplant recipients were obtained at 2 timepoints: pre-transplantation (n = 40) and post-transplantation (n = 40). Of the 40 samples at each timepoint, 23 were IRI+ and 17 were IRI-. All samples were sequenced on the Illumina HiSeq3000 platform.
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| Contributor(s) |
Ahn R, Sosa R |
| Citation(s) |
32426849 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| L60 MD011903 |
Immune Mechanisms of Hispanic Health Disparities in Liver Transplantation |
LOAN REPAYMENT APPLICATIONS |
Rebecca A Sosa |
| P01 AI120944 |
Innate-Adaptive Immunoregulation in Liver Transplant Ischemia/Reperfusion Injury |
UNIVERSITY OF CALIFORNIA LOS ANGELES |
Jerzy W Kupiec-Weglinski |
| T32 CA009120 |
UCLA Tumor Immunology Institutional Traning Grant |
UNIVERSITY OF CALIFORNIA LOS ANGELES |
MICHAEL A TEITELL |
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| Submission date |
Jun 02, 2020 |
| Last update date |
Aug 07, 2020 |
| Contact name |
Richard Sungho Ahn |
| Organization name |
University of California Los Angeles
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| Department |
Microbiology, Immunology, and Molecular Genetics
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| Street address |
611 Charles E. Young Drive East, UCLA, Boyer Hall, 510C
|
| City |
Los Angeles |
| State/province |
CA |
| ZIP/Postal code |
90095 |
| Country |
USA |
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| Platforms (1) |
| GPL21290 |
Illumina HiSeq 3000 (Homo sapiens) |
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| Samples (80)
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| Relations |
| BioProject |
PRJNA636764 |
| SRA |
SRP265616 |
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