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| Status |
Public on Mar 12, 2009 |
| Title |
Expression data from Arabidopsis seed compartments at the mature green stage. |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Expression profiling by array
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| Summary |
We collected mature green seed compartments from 7 micron paraffin sections using the Leica LMD6000 system in order to identify the mRNAs present in different compartments from seeds containing mature green-stage embryos. For the purposes of this study we captured 6 compartments: embryo proper, micropylar endosperm, cellularized peripherial endosperm, chalazal endosperm, chalazal seed coat and seed coat, as well sets of serial sections encompassing the entire mature green stage seed.
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| Overall design |
Mature green stage seed compartments were isolated using the LMD6000 system. Total RNA was amplified and hybridized with Affymetrix ATH1 Arabidopsis array for 14 samples (embryo proper, micropylar endosperm, peripherial endosperm, chalazal endosperm, chalazal seed coat, seed coat and whole seeds, 2 biological replicates each).
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| Contributor(s) |
Stone SL, Pelletier J, Kirkbride RC, Belmonte MF, Fei J, Hashimoto MM, Cheng C, Golberg RB, Harada JJ |
| Citation(s) |
23319655, 20385809 |
| Submission date |
Mar 10, 2009 |
| Last update date |
Aug 28, 2018 |
| Contact name |
John J Harada |
| E-mail(s) |
jjharada@ucdavis.edu
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| Phone |
530-752-0673
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| Organization name |
University of California, Davis
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| Department |
Plant Biology
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| Lab |
Harada
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| Street address |
One Shields Avenue
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| City |
Davis |
| State/province |
CA |
| ZIP/Postal code |
95616 |
| Country |
USA |
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| Platforms (1) |
| GPL198 |
[ATH1-121501] Affymetrix Arabidopsis ATH1 Genome Array |
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| Samples (14)
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| GSM378733 |
Laser capture microdissected (LCM) embryo proper at the mature green stage, biological replicate 1 |
| GSM378734 |
Laser capture microdissected (LCM) embryo proper at the mature green stage, biological replicate 2 |
| GSM378735 |
Laser capture microdissected (LCM) micropylar endosperm at the mature green stage, biological replicate 1 |
| GSM378736 |
Laser capture microdissected (LCM) micropylar endosperm at the mature green stage, biological replicate 2 |
| GSM378737 |
Laser capture microdissected (LCM) peripherial endosperm at the mature green stage, biological replicate 1 |
| GSM378738 |
Laser capture microdissected (LCM) peripheral endosperm at the mature green stage, biological replicate 2 |
| GSM378739 |
Laser capture microdissected (LCM) chalazal endosperm at the mature green stage, biological replicate 1 |
| GSM378740 |
Laser capture microdissected (LCM) chalazal endosperm at the mature green stage, biological replicate 2 |
| GSM378741 |
Laser capture microdissected (LCM) chalazal seed coat at the mature green stage, biological replicate 1 |
| GSM378742 |
Laser capture microdissected (LCM) chalazal seed coat at the mature green stage, biological replicate 2 |
| GSM378743 |
Laser capture microdissected (LCM) seed coat at the mature green stage, biological replicate 1 |
| GSM378744 |
Laser capture microdissected (LCM) seed coat at the mature green stage, biological replicate 2 |
| GSM378745 |
Laser capture microdissected (LCM) whole seeds at the mature green stage, biological replicate 1 |
| GSM378746 |
Laser capture microdissected (LCM) whole seeds at the mature green stage, biological replicate 2 |
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| This SubSeries is part of SuperSeries: |
| GSE12404 |
Expression data from Arabidopsis Seed Compartments at 5 discrete stages of development |
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| Relations |
| BioProject |
PRJNA114971 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE15165_RAW.tar |
27.4 Mb |
(http)(custom) |
TAR (of CEL, CHP) |
| Processed data included within Sample table |
| Processed data provided as supplementary file |
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