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| Status |
Public on Feb 08, 2021 |
| Title |
Cell-penetrant SELENOT mimetic protects dopaminergic neurons and ameliorates motor function |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Parkinson’s disease (PD) is a neurodegenerative disorder characterized by motor dysfunction for which there is an unmet need for better treatment options. Although oxidative stress is a common feature of neurodegenerative diseases, notably PD, there is currently no efficient therapeutic strategy able to tackle this system-wide pathophysiological process. Based on our previous observations of the potent antioxidant and neuroprotective activity of SELENOT, a vital thioredoxin-like selenoprotein, we designed the small peptide PSELT from its redox active site in order to determine its potential polyfunctional activity in PD models. PSELT proved to site in order to be protective for neurotoxin-treated dopaminergic neurons and fibers against oxidative stress determine its potential polyfunctional activity in PD models. PSELT proved to be protective for neurotoxin-treated dopaminergic neurons and fibers against oxidative stress and cell death. We demonstrate that PSELT is cell-permeable and acts in different subcellular compartments of dopaminergic neurons where oxidative stress exerts its deleterious effects. This protective activity prevented neurodegeneration and restored phosphorylated tyrosine hydroxylase levels, leading to improved motor skills in rodent models of PD
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| Overall design |
The human SH-SY5Y neuroblastoma cell line treated or not with 500 μM or 1 mM MPP+ for 36 h in the presence or absence of PSELT (10 μM).
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| Contributor(s) |
Elkahloun AG, Eiden LE, Anouar Y |
| Citation(s) |
33486153 |
| Submission date |
Jun 04, 2020 |
| Last update date |
Feb 08, 2021 |
| Contact name |
abdel G Elkahloun |
| E-mail(s) |
abdel@mail.nih.gov
|
| Phone |
301 402 3170
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| Organization name |
NHGRI-NIH
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| Lab |
MICROARRAY CORE
|
| Street address |
50, SOUTH DRIVE
|
| City |
BETHESDA |
| ZIP/Postal code |
20892 |
| Country |
USA |
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| Platforms (1) |
| GPL23159 |
[Clariom_S_Human] Affymetrix Clariom S Assay, Human (Includes Pico Assay) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA637314 |
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