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| Status |
Public on Jan 22, 2010 |
| Title |
RNA Expression Profiling of Lung Tissue Identifies Mutually Distinct Molecular Signatures in PAH and PH Secondary to IPF |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Idiopathic pulmonary arterial hypertension (PAH) is a life-threatening condition characterized by pulmonary arteriolar remodeling, and is frequently associated with right heart failure. This study identifies significant novel biological changes in eight genes and several genetic pathways, that were likely to contribute to the pathogenesis of PAH. We also demonstrate that PAH and PH secondary to idiopathic pulmonary fibrosis (IPF) are characterized by distinct gene expression signatures, implying distinct pathophysiological mechanisms.
Keywords: disease versus control
Keywords: Expression profiling by array
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| Overall design |
Fresh frozen lung tissue specimens from PAH subjects (n=18), IPF subjects with secondary PH (n=8), and normal controls (n=13) were obtained from the University of Pittsburgh Health Sciences Tissue Bank following approval by the University of Pittsburgh Institutional Review Board.
Total RNA was isolated from lung tissue using Trizol (Invitrogen) and purified using the RNeasy Mini kit (Qiagen). RNA quantity and quality was assessed using Agilent Nanodrop and Agilent 2100 Bioanalyzer, respectively. 500 ng of total RNA meeting was Cy3 labeled using the Agilent Low RNA Input Linear Amplification Kit PLUS (One-Color). After purification and fragmentation, aliquots of each sample were hybridized to an Agilent Whole Human Genome 4 X 44K microarray, sequentially washed, and scanned on an Agilent DNA microarray scanner.
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| Contributor(s) |
Rajkumar R, Konishi K, Ishizawar D, Wiechert A, Richards T, Kaminski N, Ahmad F |
| Citation(s) |
20081107 |
| Submission date |
Mar 11, 2009 |
| Last update date |
Jan 23, 2019 |
| Contact name |
Ferhaan Ahmad |
| E-mail(s) |
ferhaan-ahmad@uiowa.edu
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| Organization name |
University of Iowa
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| Department |
Internal Medicine
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| Street address |
100 Newton Road, 1191D ML
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| City |
Iowa City |
| State/province |
IA |
| ZIP/Postal code |
52242 |
| Country |
USA |
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| Platforms (1) |
| GPL6480 |
Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Probe Name version) |
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| Samples (39)
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| GSM379319 |
control, biological rep4 |
| GSM379320 |
control, biological rep5 |
| GSM379321 |
control, biological rep6 |
| GSM379322 |
control, biological rep7 |
| GSM379323 |
control, biological rep8 |
| GSM379324 |
control, biological rep9 |
| GSM379325 |
control, biological rep10 |
| GSM379326 |
control, biological rep11 |
| GSM379327 |
control, biological rep12 |
| GSM379328 |
control, biological rep13 |
| GSM379329 |
IPF with PH, biological rep1 |
| GSM379330 |
IPF with PH, biological rep2 |
| GSM379331 |
IPF with PH, biological rep3 |
| GSM379332 |
IPF with PH, biological rep4 |
| GSM379333 |
IPF with PH, biological rep5 |
| GSM379334 |
IPF with PH, biological rep6 |
| GSM379335 |
IPF with PH, biological rep7 |
| GSM379336 |
IPF with PH, biological rep8 |
| GSM379337 |
PAH, biological rep1 |
| GSM379338 |
PAH, biological rep2 |
| GSM379339 |
PHA, biological rep3 |
| GSM379340 |
PHA, biological rep4 |
| GSM379341 |
PHA, biological rep5 |
| GSM379342 |
PHA, biological rep6 |
| GSM379343 |
PHA, biological rep7 |
| GSM379344 |
PHA, biological rep8 |
| GSM379345 |
PHA, biological rep9 |
| GSM379346 |
PHA, biological rep10 |
| GSM379347 |
PHA, biological rep11 |
| GSM379348 |
PHA, biological rep12 |
| GSM379349 |
PHA, biological rep13 |
| GSM379350 |
PHA, biological rep14 |
| GSM379351 |
PHA, biological rep15 |
| GSM379352 |
PHA, biological rep16 |
| GSM379353 |
PHA, biological rep17 |
| GSM379354 |
PHA, biological rep18 |
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| Relations |
| BioProject |
PRJNA114953 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE15197_RAW.tar |
307.1 Mb |
(http)(custom) |
TAR (of TXT) |
| Processed data included within Sample table |
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