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| Status |
Public on Jun 01, 2010 |
| Title |
Identification and validation of NOL5A and RPS2 as potential therapeutic targtes in colorectal cancer |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Identification and validation of NOL5A and RPS2 as potential therapeutic targets in colorectal cancer using a functional genomics approach.
To identify potential therapeutic targets for colorectal cancer, we first assessed the functional and molecular consequences of RNAi mediated silencing of candidate genes derived from previously performed gene expression analyses. We then generated gene expression signatures after RNAi against HMGA1, RRM2, TACSTD2, RPS2, and NOL5A.
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| Overall design |
To assess the consequences of silencing specific genes on global gene expression levels, triplicate transfections were independently performed. Cells for lyzed 48 hours or 72 hours after transfection (depending on the target gene), and total RNA was isolated for each transfection. For each sample, one array experiment was subsequently performed.
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| Contributor(s) |
Grade M, Beissbarth T, Caplen N, Ried T |
| Citation(s) |
20473941 |
| Submission date |
Mar 12, 2009 |
| Last update date |
Feb 22, 2018 |
| Contact name |
Marian Grade |
| E-mail(s) |
marian.grade@gmail.com
|
| Organization name |
University Medical Center Goettingen, Georg-August-University
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| Department |
Surgery
|
| Street address |
Robert-Koch-Str. 40
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| City |
Goettingen |
| ZIP/Postal code |
37075 |
| Country |
Germany |
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| Platforms (1) |
| GPL4133 |
Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Feature Number version) |
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| Samples (51)
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| Relations |
| BioProject |
PRJNA116453 |
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