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| Status |
Public on Jun 01, 2009 |
| Title |
Gene expression differences between TAF4b-KO, Het and WT ovaries at 3 weeks of age |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
The rapid decline of ovarian function in TAF4b-null mice begins in early postnatal life and follicle depletion is completed by sixteen weeks.
To uncover differences in gene expression that may underlie accelerated ovarian aging, we compared genome-wide expression profiles of three week old, pre-pubescent TAF4b-null and wild-type ovaries.
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| Overall design |
Total RNA from 9, 3-week-old mice (3 wild-type, 3 TAF4b-heterozygous, 3 TAF4b-null) was obtained as described as above. RNA quality was checked using a Bioanalyzer, and concentration determined using a Nanodrop.
300 ng of each RNA sample was used in the Affymetrix Whole-transcript Sense Target Labeling Assay (Rev 3) followed by hybridization to a GeneChip® Mouse Gene 1.0 ST Array. 9 GeneChips were used to provide biological triplicates of each genotype.
The Affymetrix Expression Console (v 1.1) was used to normalize data and determine signal intensity (RMA-Sketch).
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| Contributor(s) |
Lovasco L |
| Citation(s) |
19684329 |
| Submission date |
Mar 13, 2009 |
| Last update date |
Mar 04, 2019 |
| Contact name |
Lindsay Lovasco |
| E-mail(s) |
lindsay_lovasco@brown.edu
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| Organization name |
Brown University
|
| Department |
MCB
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| Lab |
Freiman Lab
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| Street address |
70 Ship St.
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| City |
Providence |
| State/province |
RI |
| ZIP/Postal code |
02903 |
| Country |
USA |
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| Platforms (1) |
| GPL6246 |
[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA116421 |
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